1 Ocak 2021 Dergi makalesi Açık Erişim

Kayihan, Doga Selin; Kayihan, Ceyhun; Ciftci, Yelda Ozden

Citation Style Language JSON

{
  "DOI": "10.1080/15226514.2020.1786795", 
  "abstract": "Plants encounter many environmental factors such as low and high temperatures during phytoremediation processes. In this study, our aim was to produce the transgenic tobacco plants by using a newly characterized bacterial nitroreductase,Ntr, which was active at a broad range temperature in order to detoxify 2,4-dinitrotoluene (2,4-DNT) at lower temperature. The presence ofNtrand its heterologous expression was verified in T1 transgenic plants and their growing ability were determined under toxic amount of 2,4-DNT (35 mu M). Fresh weight and dry weight of transgenic plants were significantly higher than wild type (WT) under toxic 2,4-DNT at 22 degrees C, indicating higher growth capacity of the transgenics. Transgenic plants also showed a higher tolerance than WT when exposed to 2,4-DNT at 15 degrees C. Moreover, transformation rate of 2,4-DNT was gradually decreased through decreasing temperatures in WT media, however, it was increased through decreasing temperatures in transgenic plant TR3-25 media and it had the highest transformation rate (54%) of 2,4-DNT at 4 degrees C. Correlatively, 2,4-DNT treatment at 4 degrees C led to a significant decrease in H(2)O(2)level in transgenic plants. Thus, transgenic plants overexpressing nitroreductase might have an important advantage for phytoremediation of toxic nitroaromatic compounds in field applications at low temperatures.", 
  "author": [
    {
      "family": "Kayihan", 
      "given": " Doga Selin"
    }, 
    {
      "family": "Kayihan", 
      "given": " Ceyhun"
    }, 
    {
      "family": "Ciftci", 
      "given": " Yelda Ozden"
    }
  ], 
  "container_title": "INTERNATIONAL JOURNAL OF PHYTOREMEDIATION", 
  "id": "8035", 
  "issue": "1", 
  "issued": {
    "date-parts": [
      [
        2021, 
        1, 
        1
      ]
    ]
  }, 
  "page": "1-9", 
  "title": "Transgenic tobacco plants overexpressing a cold-adaptive nitroreductase gene exhibited enhanced 2,4-dinitrotoluene detoxification rate at low temperature", 
  "type": "article-journal", 
  "volume": "23"
}