Dergi makalesi Açık Erişim
Percin, Isik; Khalaf, Rushd; Brand, Bastian; Morbidelli, Massimo; Gezici, Orhan
<?xml version='1.0' encoding='UTF-8'?> <record xmlns="http://www.loc.gov/MARC21/slim"> <leader>00000nam##2200000uu#4500</leader> <controlfield tag="001">78327</controlfield> <datafield tag="540" ind1=" " ind2=" "> <subfield code="a">Creative Commons Attribution</subfield> <subfield code="u">http://www.opendefinition.org/licenses/cc-by</subfield> </datafield> <datafield tag="909" ind1="C" ind2="4"> <subfield code="v">1386</subfield> <subfield code="p">JOURNAL OF CHROMATOGRAPHY A</subfield> <subfield code="c">13-21</subfield> </datafield> <controlfield tag="005">20210316050507.0</controlfield> <datafield tag="520" ind1=" " ind2=" "> <subfield code="a">A new strong cation exchanger (SCX) monolithic column was synthesized by at-line surface modification of a cryogel prepared by copolymerization of 2-hydroxyethylmethacrylate (HEMA) and glycidyl-methacrylate (GMA). Sodium salt of 3-Mercaptopropane sulfonic acid (3-MPS) was used as the ligand to transform the surface of the monolith into a strong cation exchanger. The obtained material was characterized in terms of elemental analysis, infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Brunauer-Emmett-Teller (BET) N-2 adsorption, and used as a stationary phase for strong-cation exchange chromatography of some proteins, such as a-chymotrypsinogen, cytochrome c and lysozyme. Water permeability of the column was calculated according to Darcy's law (2.66 x 10(-13) m(2)). The performance of the monolithic cryogel column was evaluated on the basis of Height Equivalent to a Theoretical Plate (HETP). Retention behavior of the studied proteins was modeled on the basis of Yamamoto model to understand the role of the ion-exchange mechanism in retention behaviors. The considered proteins were successfully separated, and the obtained chromatogram was compared with that obtained with a non-functionalized cryogel column. (c) 2015 Elsevier B.V. All rights reserved.</subfield> </datafield> <datafield tag="245" ind1=" " ind2=" "> <subfield code="a">Strong cation-exchange chromatography of proteins on a sulfoalkylated monolithic cryogel</subfield> </datafield> <datafield tag="909" ind1="C" ind2="O"> <subfield code="p">user-tubitak-destekli-proje-yayinlari</subfield> <subfield code="o">oai:zenodo.org:78327</subfield> </datafield> <datafield tag="542" ind1=" " ind2=" "> <subfield code="l">open</subfield> </datafield> <datafield tag="100" ind1=" " ind2=" "> <subfield code="a">Percin, Isik</subfield> </datafield> <datafield tag="980" ind1=" " ind2=" "> <subfield code="a">user-tubitak-destekli-proje-yayinlari</subfield> </datafield> <datafield tag="856" ind1="4" ind2=" "> <subfield code="z">md5:08b0dd81db05341ac9d3aa2771289317</subfield> <subfield code="s">208</subfield> <subfield code="u">https://aperta.ulakbim.gov.trrecord/78327/files/bib-dceb4c43-c9e4-48ab-80f7-3310db55582b.txt</subfield> </datafield> <datafield tag="024" ind1=" " ind2=" "> <subfield code="2">doi</subfield> <subfield code="a">10.1016/j.chroma.2015.01.075</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">ETH, Inst Chem & Bioengn, CH-8093 Zurich, Switzerland</subfield> <subfield code="a">Khalaf, Rushd</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">ETH, Inst Chem & Bioengn, CH-8093 Zurich, Switzerland</subfield> <subfield code="a">Brand, Bastian</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="u">ETH, Inst Chem & Bioengn, CH-8093 Zurich, Switzerland</subfield> <subfield code="a">Morbidelli, Massimo</subfield> </datafield> <datafield tag="700" ind1=" " ind2=" "> <subfield code="a">Gezici, Orhan</subfield> </datafield> <datafield tag="650" ind1="1" ind2="7"> <subfield code="a">cc-by</subfield> <subfield code="2">opendefinition.org</subfield> </datafield> <datafield tag="980" ind1=" " ind2=" "> <subfield code="a">publication</subfield> <subfield code="b">article</subfield> </datafield> <datafield tag="260" ind1=" " ind2=" "> <subfield code="c">2015-01-01</subfield> </datafield> </record>
Görüntülenme | 17 |
İndirme | 4 |
Veri hacmi | 832 Bytes |
Tekil görüntülenme | 15 |
Tekil indirme | 4 |