1 Ocak 2015 Dergi makalesi Açık Erişim

Percin, Isik; Khalaf, Rushd; Brand, Bastian; Morbidelli, Massimo; Gezici, Orhan

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/78327</identifier>
  <creators>
    <creator>
      <creatorName>Percin, Isik</creatorName>
      <givenName>Isik</givenName>
      <familyName>Percin</familyName>
    </creator>
    <creator>
      <creatorName>Khalaf, Rushd</creatorName>
      <givenName>Rushd</givenName>
      <familyName>Khalaf</familyName>
      <affiliation>ETH, Inst Chem &amp; Bioengn, CH-8093 Zurich, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Brand, Bastian</creatorName>
      <givenName>Bastian</givenName>
      <familyName>Brand</familyName>
      <affiliation>ETH, Inst Chem &amp; Bioengn, CH-8093 Zurich, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Morbidelli, Massimo</creatorName>
      <givenName>Massimo</givenName>
      <familyName>Morbidelli</familyName>
      <affiliation>ETH, Inst Chem &amp; Bioengn, CH-8093 Zurich, Switzerland</affiliation>
    </creator>
    <creator>
      <creatorName>Gezici, Orhan</creatorName>
      <givenName>Orhan</givenName>
      <familyName>Gezici</familyName>
    </creator>
  </creators>
  <titles>
    <title>Strong Cation-Exchange Chromatography Of Proteins On A Sulfoalkylated Monolithic Cryogel</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2015</publicationYear>
  <dates>
    <date dateType="Issued">2015-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/78327</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.chroma.2015.01.075</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">A new strong cation exchanger (SCX) monolithic column was synthesized by at-line surface modification of a cryogel prepared by copolymerization of 2-hydroxyethylmethacrylate (HEMA) and glycidyl-methacrylate (GMA). Sodium salt of 3-Mercaptopropane sulfonic acid (3-MPS) was used as the ligand to transform the surface of the monolith into a strong cation exchanger. The obtained material was characterized in terms of elemental analysis, infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Brunauer-Emmett-Teller (BET) N-2 adsorption, and used as a stationary phase for strong-cation exchange chromatography of some proteins, such as a-chymotrypsinogen, cytochrome c and lysozyme. Water permeability of the column was calculated according to Darcy's law (2.66 x 10(-13) m(2)). The performance of the monolithic cryogel column was evaluated on the basis of Height Equivalent to a Theoretical Plate (HETP). Retention behavior of the studied proteins was modeled on the basis of Yamamoto model to understand the role of the ion-exchange mechanism in retention behaviors. The considered proteins were successfully separated, and the obtained chromatogram was compared with that obtained with a non-functionalized cryogel column. (c) 2015 Elsevier B.V. All rights reserved.</description>
  </descriptions>
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