1 Ocak 2015 Dergi makalesi Açık Erişim

Percin, Isik; Khalaf, Rushd; Brand, Bastian; Morbidelli, Massimo; Gezici, Orhan

Citation Style Language JSON

{
  "DOI": "10.1016/j.chroma.2015.01.075", 
  "abstract": "A new strong cation exchanger (SCX) monolithic column was synthesized by at-line surface modification of a cryogel prepared by copolymerization of 2-hydroxyethylmethacrylate (HEMA) and glycidyl-methacrylate (GMA). Sodium salt of 3-Mercaptopropane sulfonic acid (3-MPS) was used as the ligand to transform the surface of the monolith into a strong cation exchanger. The obtained material was characterized in terms of elemental analysis, infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Brunauer-Emmett-Teller (BET) N-2 adsorption, and used as a stationary phase for strong-cation exchange chromatography of some proteins, such as a-chymotrypsinogen, cytochrome c and lysozyme. Water permeability of the column was calculated according to Darcy's law (2.66 x 10(-13) m(2)). The performance of the monolithic cryogel column was evaluated on the basis of Height Equivalent to a Theoretical Plate (HETP). Retention behavior of the studied proteins was modeled on the basis of Yamamoto model to understand the role of the ion-exchange mechanism in retention behaviors. The considered proteins were successfully separated, and the obtained chromatogram was compared with that obtained with a non-functionalized cryogel column. (c) 2015 Elsevier B.V. All rights reserved.", 
  "author": [
    {
      "family": "Percin", 
      "given": " Isik"
    }, 
    {
      "family": "Khalaf", 
      "given": " Rushd"
    }, 
    {
      "family": "Brand", 
      "given": " Bastian"
    }, 
    {
      "family": "Morbidelli", 
      "given": " Massimo"
    }, 
    {
      "family": "Gezici", 
      "given": " Orhan"
    }
  ], 
  "container_title": "JOURNAL OF CHROMATOGRAPHY A", 
  "id": "78327", 
  "issued": {
    "date-parts": [
      [
        2015, 
        1, 
        1
      ]
    ]
  }, 
  "page": "13-21", 
  "title": "Strong cation-exchange chromatography of proteins on a sulfoalkylated monolithic cryogel", 
  "type": "article-journal", 
  "volume": "1386"
}