1 Ocak 2014 Dergi makalesi Açık Erişim

Sari, A. Nihal; Kacan, Meltem; Unsal, Demet; Firat, Seyhan Sahan; Buharalioglu, C. Kemel; Vezir, Ozden; Korkmaz, Belma; Cuez, Tuba; Canacankatan, Necmiye; Sucu, Nehir; Ayaz, Lokman; Gumus, Lulufer Tamer; Gorur, Aysegul; Tunctan, Bahar

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        "affiliation": "Mersin Univ, Fac Pharm, Dept Pharmacol, TR-33169 Mersin, Turkey", 
        "name": "Sari, A. Nihal"
      }, 
      {
        "affiliation": "Mersin Univ, Fac Pharm, Dept Pharmacol, TR-33169 Mersin, Turkey", 
        "name": "Kacan, Meltem"
      }, 
      {
        "affiliation": "Mersin Univ, Fac Pharm, Dept Pharmacol, TR-33169 Mersin, Turkey", 
        "name": "Unsal, Demet"
      }, 
      {
        "affiliation": "Mersin Univ, Fac Pharm, Dept Pharmacol, TR-33169 Mersin, Turkey", 
        "name": "Firat, Seyhan Sahan"
      }, 
      {
        "affiliation": "Mersin Univ, Fac Pharm, Dept Pharmacol, TR-33169 Mersin, Turkey", 
        "name": "Buharalioglu, C. Kemel"
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      {
        "affiliation": "Mersin Univ, Fac Med, Dept Cardiovasc Surg, TR-33169 Mersin, Turkey", 
        "name": "Vezir, Ozden"
      }, 
      {
        "affiliation": "Mersin Univ, Fac Pharm, Dept Pharmacol, TR-33169 Mersin, Turkey", 
        "name": "Korkmaz, Belma"
      }, 
      {
        "affiliation": "Mersin Univ, Fac Pharm, Dept Pharmacol, TR-33169 Mersin, Turkey", 
        "name": "Cuez, Tuba"
      }, 
      {
        "affiliation": "Mersin Univ, Fac Pharm, Dept Biochem, TR-33169 Mersin, Turkey", 
        "name": "Canacankatan, Necmiye"
      }, 
      {
        "affiliation": "Mersin Univ, Fac Med, Dept Cardiovasc Surg, TR-33169 Mersin, Turkey", 
        "name": "Sucu, Nehir"
      }, 
      {
        "affiliation": "Mersin Univ, Fac Med, Dept Med Biochem, TR-33169 Mersin, Turkey", 
        "name": "Ayaz, Lokman"
      }, 
      {
        "affiliation": "Mersin Univ, Fac Med, Dept Med Biochem, TR-33169 Mersin, Turkey", 
        "name": "Gumus, Lulufer Tamer"
      }, 
      {
        "affiliation": "Mersin Univ, Fac Pharm, Dept Biochem, TR-33169 Mersin, Turkey", 
        "name": "Gorur, Aysegul"
      }, 
      {
        "affiliation": "Mersin Univ, Fac Pharm, Dept Pharmacol, TR-33169 Mersin, Turkey", 
        "name": "Tunctan, Bahar"
      }
    ], 
    "description": "The small G protein RhoA and its downstream effector Rho-kinase play an important role in various physiopathological processes including ischemia/reperfusion (I/R) injury. Reactive oxygen and nitrogen species produced by iNOS and NADPH oxidase are important mediators of inflammation and organ injury following an initial localized I/R event. The aim of this study was to determine whether RhoA/Rho-kinase signaling pathway increases the expression and activity of MEK1, ERK1/2, iNOS, gp91(phox), and p47(phox), and peroxynitrite formation which result in oxidative/nitrosative stress and inflammation leading to hindlimb PR-induced injury in kidney as a distant organ and;,gastrocnemius muscle as a target organ. I/R-induced distant and target organ injury was performed by using the rat hindlimb tourniquet model, I/R caused an increase in the expression and/or activity of RhoA, MEK1, ERK1/2, iNOS, gp91(phox), p47(phox), and 3-nitrotyrosine and nitrotyrosine levels in the tissues. Although Rho-kinase activity was increased by I/R in the kidney, its activity was decreased in the muscle. Serum and tissue MDA levels and MPO activity were increased following I/R. PR also caused an increase in SOD and catalase activities associated with decreased GSH levels in the tissues. Y-27632, a selective Rho-kinase inhibitor, (100 mu g/kg, i.p.; 1 h before reperfusion) prevented the I/R-induced changes except Rho-kinase activity in the muscle. These results suggest that activation of RhoA/Rho-kinase/MEK1/ERK1/2/iNOS pathway associated with oxidative/nitrosative stress and inflammation contributes to hindlimb PR-induced distant organ injury in rats. It also seems that hindlimb I/R induces target organ injury via upregulation of RhoA/MEK1/ERK1/2/iNOS pathway associated with decreased Rho-kinase activity. (C) 2013 Elsevier B.V. All rights reserved.", 
    "doi": "10.1016/j.ejphar.2013.11.027", 
    "has_grant": false, 
    "journal": {
      "pages": "234-245", 
      "title": "EUROPEAN JOURNAL OF PHARMACOLOGY", 
      "volume": "723"
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    "license": {
      "id": "cc-by"
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    "publication_date": "2014-01-01", 
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    "resource_type": {
      "subtype": "article", 
      "title": "Dergi makalesi", 
      "type": "publication"
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    "title": "Contribution of RhoA/Rho-kinase/MEK1/ERK1/2/iNOS pathway to ischemia/reperfusion-induced oxidative/nitrosative stress and inflammation leading to distant and target organ injury in rats"
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