1 Ocak 2014 Dergi makalesi Açık Erişim

Sari, A. Nihal; Kacan, Meltem; Unsal, Demet; Firat, Seyhan Sahan; Buharalioglu, C. Kemel; Vezir, Ozden; Korkmaz, Belma; Cuez, Tuba; Canacankatan, Necmiye; Sucu, Nehir; Ayaz, Lokman; Gumus, Lulufer Tamer; Gorur, Aysegul; Tunctan, Bahar

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{
  "DOI": "10.1016/j.ejphar.2013.11.027", 
  "abstract": "The small G protein RhoA and its downstream effector Rho-kinase play an important role in various physiopathological processes including ischemia/reperfusion (I/R) injury. Reactive oxygen and nitrogen species produced by iNOS and NADPH oxidase are important mediators of inflammation and organ injury following an initial localized I/R event. The aim of this study was to determine whether RhoA/Rho-kinase signaling pathway increases the expression and activity of MEK1, ERK1/2, iNOS, gp91(phox), and p47(phox), and peroxynitrite formation which result in oxidative/nitrosative stress and inflammation leading to hindlimb PR-induced injury in kidney as a distant organ and;,gastrocnemius muscle as a target organ. I/R-induced distant and target organ injury was performed by using the rat hindlimb tourniquet model, I/R caused an increase in the expression and/or activity of RhoA, MEK1, ERK1/2, iNOS, gp91(phox), p47(phox), and 3-nitrotyrosine and nitrotyrosine levels in the tissues. Although Rho-kinase activity was increased by I/R in the kidney, its activity was decreased in the muscle. Serum and tissue MDA levels and MPO activity were increased following I/R. PR also caused an increase in SOD and catalase activities associated with decreased GSH levels in the tissues. Y-27632, a selective Rho-kinase inhibitor, (100 mu g/kg, i.p.; 1 h before reperfusion) prevented the I/R-induced changes except Rho-kinase activity in the muscle. These results suggest that activation of RhoA/Rho-kinase/MEK1/ERK1/2/iNOS pathway associated with oxidative/nitrosative stress and inflammation contributes to hindlimb PR-induced distant organ injury in rats. It also seems that hindlimb I/R induces target organ injury via upregulation of RhoA/MEK1/ERK1/2/iNOS pathway associated with decreased Rho-kinase activity. (C) 2013 Elsevier B.V. All rights reserved.", 
  "author": [
    {
      "family": "Sari", 
      "given": " A. Nihal"
    }, 
    {
      "family": "Kacan", 
      "given": " Meltem"
    }, 
    {
      "family": "Unsal", 
      "given": " Demet"
    }, 
    {
      "family": "Firat", 
      "given": " Seyhan Sahan"
    }, 
    {
      "family": "Buharalioglu", 
      "given": " C. Kemel"
    }, 
    {
      "family": "Vezir", 
      "given": " Ozden"
    }, 
    {
      "family": "Korkmaz", 
      "given": " Belma"
    }, 
    {
      "family": "Cuez", 
      "given": " Tuba"
    }, 
    {
      "family": "Canacankatan", 
      "given": " Necmiye"
    }, 
    {
      "family": "Sucu", 
      "given": " Nehir"
    }, 
    {
      "family": "Ayaz", 
      "given": " Lokman"
    }, 
    {
      "family": "Gumus", 
      "given": " Lulufer Tamer"
    }, 
    {
      "family": "Gorur", 
      "given": " Aysegul"
    }, 
    {
      "family": "Tunctan", 
      "given": " Bahar"
    }
  ], 
  "container_title": "EUROPEAN JOURNAL OF PHARMACOLOGY", 
  "id": "65705", 
  "issued": {
    "date-parts": [
      [
        2014, 
        1, 
        1
      ]
    ]
  }, 
  "page": "234-245", 
  "title": "Contribution of RhoA/Rho-kinase/MEK1/ERK1/2/iNOS pathway to ischemia/reperfusion-induced oxidative/nitrosative stress and inflammation leading to distant and target organ injury in rats", 
  "type": "article-journal", 
  "volume": "723"
}