1 Ocak 2014 Dergi makalesi Açık Erişim

Varga, Zoltan V.; Zvara, Agnes; Farago, Nora; Kocsis, Gabriella F.; Pipicz, Marton; Gaspar, Renata; Bencsik, Peter; Goerbe, Aniko; Csonka, Csaba; Puskas, Laszlo G.; Thum, Thomas; Csont, Tamas; Ferdinandy, Peter

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  <dc:creator>Varga, Zoltan V.</dc:creator>
  <dc:creator>Zvara, Agnes</dc:creator>
  <dc:creator>Farago, Nora</dc:creator>
  <dc:creator>Kocsis, Gabriella F.</dc:creator>
  <dc:creator>Pipicz, Marton</dc:creator>
  <dc:creator>Gaspar, Renata</dc:creator>
  <dc:creator>Bencsik, Peter</dc:creator>
  <dc:creator>Goerbe, Aniko</dc:creator>
  <dc:creator>Csonka, Csaba</dc:creator>
  <dc:creator>Puskas, Laszlo G.</dc:creator>
  <dc:creator>Thum, Thomas</dc:creator>
  <dc:creator>Csont, Tamas</dc:creator>
  <dc:creator>Ferdinandy, Peter</dc:creator>
  <dc:date>2014-01-01</dc:date>
  <dc:description>We aimed to characterize early changes in microRNA expression in acute cardioprotection by ischemic pre- and postconditioning in rat hearts. Hearts isolated from male Wistar rats were subjected to 1) time-matched nonischemic perfusion, 2) ischemia-reperfusion (30 min of coronary occlusion and 120 min of reperfusion), 3) preconditioning (3 x 5 min of coronary occlusion) followed by ischemia-reperfusion, or 4) ischemia-reperfusion with postconditioning (6 x 10 s of global ischemia-reperfusion at the onset of reperfusion). Infarct size was significantly reduced by both interventions. Of 350 different microRNAs assessed by microarray analysis, 147-160 microRNAs showed detectable expression levels. Compared with microRNA alterations induced by ischemia-reperfusion versus time-matched nonischemic controls, five microRNAs were significantly affected by both pre- and postconditioning (microRNA-125b*, microRNA-139-3p, microRNA-320, microRNA-532-3p, and microRNA-188), four microRNAs were significantly affected by preconditioning (microRNA-487b, microRNA-139-5p, microRNA-192, and microRNA-212), and nine microRNAs were significantly affected by postconditioning (microRNA-1, microRNA let-7i, microRNA let-7e, microRNA let-7b, microRNA-181a, microRNA-208, microRNA-328, microRNA-335, and microRNA-503). Expression of randomly selected microRNAs was validated by quantitative real-time PCR. By a systematic comparison of the direction of microRNA expression changes in all groups, we identified microRNAs, specific mimics, or antagomiRs that may have pre- and postconditioning-like cardioprotective effects (protectomiRs). Transfection of selected protectomiRs (mimics of microRNA-139-5p, microRNA-125b*, microRNA let-7b, and inhibitor of microRNA-487b) into cardiac myocytes subjected to simulated ischemia-reperfusion showed a significant cytoprotective effect. This is the first demonstration that the ischemia-reperfusion-induced microRNA expression profile is significantly influenced by both pre- and postconditioning, which shows the involvement of microRNAs in cardioprotective signaling. Moreover, by analysis of microRNA expression patterns in cardioprotection by pre- and postconditioning, specific protectomiRs can be revealed as potential therapeutic tools for the treatment of ischemia-reperfusion injury.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/63873</dc:identifier>
  <dc:identifier>oai:zenodo.org:63873</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY 307(2) H216-H227</dc:source>
  <dc:title>MicroRNAs associated with ischemia-reperfusion injury and cardioprotection by ischemic pre- and postconditioning: protectomiRs</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
</oai_dc:dc>