1 Ocak 2014 Dergi makalesi Açık Erişim

Varga, Zoltan V.; Zvara, Agnes; Farago, Nora; Kocsis, Gabriella F.; Pipicz, Marton; Gaspar, Renata; Bencsik, Peter; Goerbe, Aniko; Csonka, Csaba; Puskas, Laszlo G.; Thum, Thomas; Csont, Tamas; Ferdinandy, Peter

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{
  "@context": "https://schema.org/", 
  "@id": 63873, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Varga, Zoltan V."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Hungarian Acad Sci, Biol Res Ctr, Inst Genet, H-6701 Szeged, Hungary", 
      "name": "Zvara, Agnes"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Hungarian Acad Sci, Biol Res Ctr, Inst Genet, H-6701 Szeged, Hungary", 
      "name": "Farago, Nora"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Univ Szeged, Dept Biochem, Cardiovasc Res Grp, Szeged, Hungary", 
      "name": "Kocsis, Gabriella F."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Univ Szeged, Dept Biochem, Cardiovasc Res Grp, Szeged, Hungary", 
      "name": "Pipicz, Marton"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Univ Szeged, Dept Biochem, Cardiovasc Res Grp, Szeged, Hungary", 
      "name": "Gaspar, Renata"
    }, 
    {
      "@type": "Person", 
      "name": "Bencsik, Peter"
    }, 
    {
      "@type": "Person", 
      "name": "Goerbe, Aniko"
    }, 
    {
      "@type": "Person", 
      "name": "Csonka, Csaba"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Hungarian Acad Sci, Biol Res Ctr, Inst Genet, H-6701 Szeged, Hungary", 
      "name": "Puskas, Laszlo G."
    }, 
    {
      "@type": "Person", 
      "name": "Thum, Thomas"
    }, 
    {
      "@type": "Person", 
      "name": "Csont, Tamas"
    }, 
    {
      "@type": "Person", 
      "name": "Ferdinandy, Peter"
    }
  ], 
  "datePublished": "2014-01-01", 
  "description": "We aimed to characterize early changes in microRNA expression in acute cardioprotection by ischemic pre- and postconditioning in rat hearts. Hearts isolated from male Wistar rats were subjected to 1) time-matched nonischemic perfusion, 2) ischemia-reperfusion (30 min of coronary occlusion and 120 min of reperfusion), 3) preconditioning (3 x 5 min of coronary occlusion) followed by ischemia-reperfusion, or 4) ischemia-reperfusion with postconditioning (6 x 10 s of global ischemia-reperfusion at the onset of reperfusion). Infarct size was significantly reduced by both interventions. Of 350 different microRNAs assessed by microarray analysis, 147-160 microRNAs showed detectable expression levels. Compared with microRNA alterations induced by ischemia-reperfusion versus time-matched nonischemic controls, five microRNAs were significantly affected by both pre- and postconditioning (microRNA-125b*, microRNA-139-3p, microRNA-320, microRNA-532-3p, and microRNA-188), four microRNAs were significantly affected by preconditioning (microRNA-487b, microRNA-139-5p, microRNA-192, and microRNA-212), and nine microRNAs were significantly affected by postconditioning (microRNA-1, microRNA let-7i, microRNA let-7e, microRNA let-7b, microRNA-181a, microRNA-208, microRNA-328, microRNA-335, and microRNA-503). Expression of randomly selected microRNAs was validated by quantitative real-time PCR. By a systematic comparison of the direction of microRNA expression changes in all groups, we identified microRNAs, specific mimics, or antagomiRs that may have pre- and postconditioning-like cardioprotective effects (protectomiRs). Transfection of selected protectomiRs (mimics of microRNA-139-5p, microRNA-125b*, microRNA let-7b, and inhibitor of microRNA-487b) into cardiac myocytes subjected to simulated ischemia-reperfusion showed a significant cytoprotective effect. This is the first demonstration that the ischemia-reperfusion-induced microRNA expression profile is significantly influenced by both pre- and postconditioning, which shows the involvement of microRNAs in cardioprotective signaling. Moreover, by analysis of microRNA expression patterns in cardioprotection by pre- and postconditioning, specific protectomiRs can be revealed as potential therapeutic tools for the treatment of ischemia-reperfusion injury.", 
  "headline": "MicroRNAs associated with ischemia-reperfusion injury and cardioprotection by ischemic pre- and postconditioning: protectomiRs", 
  "identifier": 63873, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "MicroRNAs associated with ischemia-reperfusion injury and cardioprotection by ischemic pre- and postconditioning: protectomiRs", 
  "url": "https://aperta.ulakbim.gov.tr/record/63873"
}