1 Ocak 2014 Dergi makalesi Açık Erişim

Varga, Zoltan V.; Zvara, Agnes; Farago, Nora; Kocsis, Gabriella F.; Pipicz, Marton; Gaspar, Renata; Bencsik, Peter; Goerbe, Aniko; Csonka, Csaba; Puskas, Laszlo G.; Thum, Thomas; Csont, Tamas; Ferdinandy, Peter

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/63873</identifier>
  <creators>
    <creator>
      <creatorName>Varga, Zoltan V.</creatorName>
      <givenName>Zoltan V.</givenName>
      <familyName>Varga</familyName>
    </creator>
    <creator>
      <creatorName>Zvara, Agnes</creatorName>
      <givenName>Agnes</givenName>
      <familyName>Zvara</familyName>
      <affiliation>Hungarian Acad Sci, Biol Res Ctr, Inst Genet, H-6701 Szeged, Hungary</affiliation>
    </creator>
    <creator>
      <creatorName>Farago, Nora</creatorName>
      <givenName>Nora</givenName>
      <familyName>Farago</familyName>
      <affiliation>Hungarian Acad Sci, Biol Res Ctr, Inst Genet, H-6701 Szeged, Hungary</affiliation>
    </creator>
    <creator>
      <creatorName>Kocsis, Gabriella F.</creatorName>
      <givenName>Gabriella F.</givenName>
      <familyName>Kocsis</familyName>
      <affiliation>Univ Szeged, Dept Biochem, Cardiovasc Res Grp, Szeged, Hungary</affiliation>
    </creator>
    <creator>
      <creatorName>Pipicz, Marton</creatorName>
      <givenName>Marton</givenName>
      <familyName>Pipicz</familyName>
      <affiliation>Univ Szeged, Dept Biochem, Cardiovasc Res Grp, Szeged, Hungary</affiliation>
    </creator>
    <creator>
      <creatorName>Gaspar, Renata</creatorName>
      <givenName>Renata</givenName>
      <familyName>Gaspar</familyName>
      <affiliation>Univ Szeged, Dept Biochem, Cardiovasc Res Grp, Szeged, Hungary</affiliation>
    </creator>
    <creator>
      <creatorName>Bencsik, Peter</creatorName>
      <givenName>Peter</givenName>
      <familyName>Bencsik</familyName>
    </creator>
    <creator>
      <creatorName>Goerbe, Aniko</creatorName>
      <givenName>Aniko</givenName>
      <familyName>Goerbe</familyName>
    </creator>
    <creator>
      <creatorName>Csonka, Csaba</creatorName>
      <givenName>Csaba</givenName>
      <familyName>Csonka</familyName>
    </creator>
    <creator>
      <creatorName>Puskas, Laszlo G.</creatorName>
      <givenName>Laszlo G.</givenName>
      <familyName>Puskas</familyName>
      <affiliation>Hungarian Acad Sci, Biol Res Ctr, Inst Genet, H-6701 Szeged, Hungary</affiliation>
    </creator>
    <creator>
      <creatorName>Thum, Thomas</creatorName>
      <givenName>Thomas</givenName>
      <familyName>Thum</familyName>
    </creator>
    <creator>
      <creatorName>Csont, Tamas</creatorName>
      <givenName>Tamas</givenName>
      <familyName>Csont</familyName>
    </creator>
    <creator>
      <creatorName>Ferdinandy, Peter</creatorName>
      <givenName>Peter</givenName>
      <familyName>Ferdinandy</familyName>
    </creator>
  </creators>
  <titles>
    <title>Micrornas Associated With Ischemia-Reperfusion Injury And Cardioprotection By Ischemic Pre- And Postconditioning: Protectomirs</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2014</publicationYear>
  <dates>
    <date dateType="Issued">2014-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/63873</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1152/ajpheart.00812.2013</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">We aimed to characterize early changes in microRNA expression in acute cardioprotection by ischemic pre- and postconditioning in rat hearts. Hearts isolated from male Wistar rats were subjected to 1) time-matched nonischemic perfusion, 2) ischemia-reperfusion (30 min of coronary occlusion and 120 min of reperfusion), 3) preconditioning (3 x 5 min of coronary occlusion) followed by ischemia-reperfusion, or 4) ischemia-reperfusion with postconditioning (6 x 10 s of global ischemia-reperfusion at the onset of reperfusion). Infarct size was significantly reduced by both interventions. Of 350 different microRNAs assessed by microarray analysis, 147-160 microRNAs showed detectable expression levels. Compared with microRNA alterations induced by ischemia-reperfusion versus time-matched nonischemic controls, five microRNAs were significantly affected by both pre- and postconditioning (microRNA-125b*, microRNA-139-3p, microRNA-320, microRNA-532-3p, and microRNA-188), four microRNAs were significantly affected by preconditioning (microRNA-487b, microRNA-139-5p, microRNA-192, and microRNA-212), and nine microRNAs were significantly affected by postconditioning (microRNA-1, microRNA let-7i, microRNA let-7e, microRNA let-7b, microRNA-181a, microRNA-208, microRNA-328, microRNA-335, and microRNA-503). Expression of randomly selected microRNAs was validated by quantitative real-time PCR. By a systematic comparison of the direction of microRNA expression changes in all groups, we identified microRNAs, specific mimics, or antagomiRs that may have pre- and postconditioning-like cardioprotective effects (protectomiRs). Transfection of selected protectomiRs (mimics of microRNA-139-5p, microRNA-125b*, microRNA let-7b, and inhibitor of microRNA-487b) into cardiac myocytes subjected to simulated ischemia-reperfusion showed a significant cytoprotective effect. This is the first demonstration that the ischemia-reperfusion-induced microRNA expression profile is significantly influenced by both pre- and postconditioning, which shows the involvement of microRNAs in cardioprotective signaling. Moreover, by analysis of microRNA expression patterns in cardioprotection by pre- and postconditioning, specific protectomiRs can be revealed as potential therapeutic tools for the treatment of ischemia-reperfusion injury.</description>
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