1 Ocak 2014 Dergi makalesi Açık Erişim

Babadagli, Mustafa Ege; Tezcan, Berna; Yilmaz, Seda Tasir; Tufan, A. Cevik

JSON-LD (schema.org)

{
  "@context": "https://schema.org/", 
  "@id": 61247, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Babadagli, Mustafa Ege"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Osmangazi Univ, Sch Med, Dept Histol & Embryol, Eskisehir, Turkey", 
      "name": "Tezcan, Berna"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ankara Univ, Inst Biotechnol, TR-06100 Ankara, Turkey", 
      "name": "Yilmaz, Seda Tasir"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Pamukkale Univ, Sch Med, Dept Histol & Embryol, Denizli, Turkey", 
      "name": "Tufan, A. Cevik"
    }
  ], 
  "datePublished": "2014-01-01", 
  "description": "C-type natriuretic peptide (CNP) signaling has been implicated as an important regulator of chondrogenic differentiation during endochondral bone development. This preliminary study further investigated the putative effectors and/or targets of CNP signaling in transforming growth factor (TGF)-beta induced in vitro chondrogenic differentiation of mesenchymal stem cells (MSCs). Previously characterized human trabecular bone derived MSCs were induced either with only TGF-beta 1 or with a combination of TGF-beta 1 and CNP in micromass culture for 10 or 20 days. Genome wide gene expression profile changes in between these two groups were analyzed on day-10 or day-20 of culture. Results revealed that there were only 7 genes, whose expression change was fourfolds or higher in TGF-beta 1 and CNP fed group in comparison to only TGF-beta 1 fed group. The up-regulated genes included matrilin-3 (MATN3), engulfment and cell motility 1 (ELMO1), CD24, and DCN1, defective in cullin neddylation 1, domain containing 1 (DCUN1D1). The down-regulated genes, on the other hand, included LIM domain kinase 2 (LIMK2), Ewing sarcoma breakpoint region 1, and guanine nucleotide binding protein (G protein), gamma 12 (GNG12). The up-regulation of MATN3 was confirmed on the basis of RT-PCR. The known literature on both CNP signaling and MATN3 function in chondrogenesis match with each other and suggest MATN3 as a putative effector and/or target of CNP signaling during this process.", 
  "headline": "Matrilin-3 as a putative effector of C-type natriuretic peptide signaling during TGF-beta induced chondrogenic differentiation of mesenchymal stem cells", 
  "identifier": 61247, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Matrilin-3 as a putative effector of C-type natriuretic peptide signaling during TGF-beta induced chondrogenic differentiation of mesenchymal stem cells", 
  "url": "https://aperta.ulakbim.gov.tr/record/61247"
}