1 Ocak 2014 Dergi makalesi Açık Erişim

Babadagli, Mustafa Ege; Tezcan, Berna; Yilmaz, Seda Tasir; Tufan, A. Cevik

Citation Style Language JSON

{
  "DOI": "10.1007/s11033-014-3448-3", 
  "abstract": "C-type natriuretic peptide (CNP) signaling has been implicated as an important regulator of chondrogenic differentiation during endochondral bone development. This preliminary study further investigated the putative effectors and/or targets of CNP signaling in transforming growth factor (TGF)-beta induced in vitro chondrogenic differentiation of mesenchymal stem cells (MSCs). Previously characterized human trabecular bone derived MSCs were induced either with only TGF-beta 1 or with a combination of TGF-beta 1 and CNP in micromass culture for 10 or 20 days. Genome wide gene expression profile changes in between these two groups were analyzed on day-10 or day-20 of culture. Results revealed that there were only 7 genes, whose expression change was fourfolds or higher in TGF-beta 1 and CNP fed group in comparison to only TGF-beta 1 fed group. The up-regulated genes included matrilin-3 (MATN3), engulfment and cell motility 1 (ELMO1), CD24, and DCN1, defective in cullin neddylation 1, domain containing 1 (DCUN1D1). The down-regulated genes, on the other hand, included LIM domain kinase 2 (LIMK2), Ewing sarcoma breakpoint region 1, and guanine nucleotide binding protein (G protein), gamma 12 (GNG12). The up-regulation of MATN3 was confirmed on the basis of RT-PCR. The known literature on both CNP signaling and MATN3 function in chondrogenesis match with each other and suggest MATN3 as a putative effector and/or target of CNP signaling during this process.", 
  "author": [
    {
      "family": "Babadagli", 
      "given": " Mustafa Ege"
    }, 
    {
      "family": "Tezcan", 
      "given": " Berna"
    }, 
    {
      "family": "Yilmaz", 
      "given": " Seda Tasir"
    }, 
    {
      "family": "Tufan", 
      "given": " A. Cevik"
    }
  ], 
  "container_title": "MOLECULAR BIOLOGY REPORTS", 
  "id": "61247", 
  "issue": "9", 
  "issued": {
    "date-parts": [
      [
        2014, 
        1, 
        1
      ]
    ]
  }, 
  "page": "5549-5555", 
  "title": "Matrilin-3 as a putative effector of C-type natriuretic peptide signaling during TGF-beta induced chondrogenic differentiation of mesenchymal stem cells", 
  "type": "article-journal", 
  "volume": "41"
}