1 Ocak 2020 Dergi makalesi Açık Erişim

Cevik, Mehtap; Gunduz, Meliha Koldemir; Deliorman, Gokce; Susleyici, Belgin

Citation Style Language JSON

{
  "DOI": "10.1007/s11033-020-05992-5", 
  "abstract": "Adipocyte death is important in obesity development. Understanding and prevention of adipocyte deaths may be a molecular approach in the treatment. In the study, we aimed to understand role of Niban gene, which acts as an anti-apoptotic molecule as a response to stress conditions, in adipocytes. 3T3-L1 adipocytes were treated with different doses of linoleic acid, hydrogen peroxide and ethanol; and proliferation of the cells examined with real time monitoring iCELLingence system. Gene expression levels were measured by q-PCR. As a response to 24h 480 mu M linoleic acid treatment, Niban gene expression was found to be higher than control group (p = 0.008), whereas 24 h 90 mM ethanol treatment was determined to be lower than control group (p = 0.008). The highest value of Niban gene expression among H2O2 treatment groups was detected in 4h 600 mu M H2O2 in comparison to control group (p = 0.008). To understand role of Niban in adipogenesis, Niban gene expressions were compared between pre-adipocytes and advanced fat accumulated adipocytes and determined to be significantly different (p = 0.042). Our results suggest that Niban might be involved in stress response process in adipocytes. However, the exact molecular role of Niban needs to be investigated in further studies.", 
  "author": [
    {
      "family": "Cevik", 
      "given": " Mehtap"
    }, 
    {
      "family": "Gunduz", 
      "given": " Meliha Koldemir"
    }, 
    {
      "family": "Deliorman", 
      "given": " Gokce"
    }, 
    {
      "family": "Susleyici", 
      "given": " Belgin"
    }
  ], 
  "container_title": "MOLECULAR BIOLOGY REPORTS", 
  "id": "6037", 
  "issue": "12", 
  "issued": {
    "date-parts": [
      [
        2020, 
        1, 
        1
      ]
    ]
  }, 
  "page": "9399-9408", 
  "title": "Alterations in niban gene expression as a response to stress conditions in 3T3-L1 adipocytes", 
  "type": "article-journal", 
  "volume": "47"
}