1 Ocak 2017 Dergi makalesi Açık Erişim

Mamedov, Tarlan; Cicek, Kader; Gulec, Burcu; Ungor, Rifat; Hasanova, Gulnara

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  <dc:creator>Mamedov, Tarlan</dc:creator>
  <dc:creator>Cicek, Kader</dc:creator>
  <dc:creator>Gulec, Burcu</dc:creator>
  <dc:creator>Ungor, Rifat</dc:creator>
  <dc:creator>Hasanova, Gulnara</dc:creator>
  <dc:date>2017-01-01</dc:date>
  <dc:description>A plant transient expression system, with eukaryotic post-translational modification machinery, offers superior efficiency, scalability, safety, and lower cost over other expression systems. However, due to aberrant N-glycosylation, this expression system may not be a suitable expression platform for proteins not carrying N-linked glycans in the native hosts. Therefore, it is crucial to develop a strategy to produce target proteins in a non-glycosylated form while preserving their native sequence, conformation and biological activity. Previously, we developed a strategy for enzymatic deglycosylation of proteins in planta by co-expressing bacterial peptide-N-glycosidase F (PNGase F). Though PNGase F removes oligosaccharides from glycosylated proteins, in so doing it causes an amino acid change due to the deamidation of asparagine to aspartate in the N-X-S/T site. Endo-beta-N-acetylglucosaminidase (EC3.2.1.96, Endo H), another deglycosylating enzyme, catalyzes cleavage between two N-Acetyl-D-glucosamine residues of the chitobiose core of N-linked glycans, leaving a single N-Acetyl-D-glucosamine residue without the concomitant deamidation of asparagine. In this study, a method for in vivo deglycosylation of recombinant proteins in plants by transient co-expression with bacterial Endo H is described for the first time. Endo H was fully active in vivo. and successfully cleaved N-linked glycans from glycoproteins were tested. In addition, unlike the glycosylated form, in vivo Endo H deglycosylated Pfs48/45 was recognized by conformational specific Pfs48/45 monoclonal antibody, in a manner similar to its PNGase F deglycosylated counterpart. Furthermore, the deglycosylated PA83 molecule produced by Endo H showed better stability than a PNGase F deglycosylated counterpart. Thus, an Endo H in vivo deglycosylation approach provides another opportunity to develop vaccine antigens, therapeutic proteins, antibodies, and industrial enzymes.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/48931</dc:identifier>
  <dc:identifier>oai:zenodo.org:48931</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>PLOS ONE 12(8)</dc:source>
  <dc:title>In vivo production of non-glycosylated recombinant proteins in Nicotiana benthamiana plants by co-expression with Endo-beta-N-acetylglucosaminidase H (Endo H) of Streptomyces plicatus</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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