1 Ocak 2017 Dergi makalesi Açık Erişim

Mamedov, Tarlan; Cicek, Kader; Gulec, Burcu; Ungor, Rifat; Hasanova, Gulnara

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{
  "@context": "https://schema.org/", 
  "@id": 48931, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Akdeniz Univ, Dept Agr Biotechnol, Antalya, Turkey", 
      "name": "Mamedov, Tarlan"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Akdeniz Univ, Dept Agr Biotechnol, Antalya, Turkey", 
      "name": "Cicek, Kader"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Akdeniz Univ, Dept Agr Biotechnol, Antalya, Turkey", 
      "name": "Gulec, Burcu"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Akdeniz Univ, Dept Agr Biotechnol, Antalya, Turkey", 
      "name": "Ungor, Rifat"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Akdeniz Univ, Dept Agr Biotechnol, Antalya, Turkey", 
      "name": "Hasanova, Gulnara"
    }
  ], 
  "datePublished": "2017-01-01", 
  "description": "A plant transient expression system, with eukaryotic post-translational modification machinery, offers superior efficiency, scalability, safety, and lower cost over other expression systems. However, due to aberrant N-glycosylation, this expression system may not be a suitable expression platform for proteins not carrying N-linked glycans in the native hosts. Therefore, it is crucial to develop a strategy to produce target proteins in a non-glycosylated form while preserving their native sequence, conformation and biological activity. Previously, we developed a strategy for enzymatic deglycosylation of proteins in planta by co-expressing bacterial peptide-N-glycosidase F (PNGase F). Though PNGase F removes oligosaccharides from glycosylated proteins, in so doing it causes an amino acid change due to the deamidation of asparagine to aspartate in the N-X-S/T site. Endo-beta-N-acetylglucosaminidase (EC3.2.1.96, Endo H), another deglycosylating enzyme, catalyzes cleavage between two N-Acetyl-D-glucosamine residues of the chitobiose core of N-linked glycans, leaving a single N-Acetyl-D-glucosamine residue without the concomitant deamidation of asparagine. In this study, a method for in vivo deglycosylation of recombinant proteins in plants by transient co-expression with bacterial Endo H is described for the first time. Endo H was fully active in vivo. and successfully cleaved N-linked glycans from glycoproteins were tested. In addition, unlike the glycosylated form, in vivo Endo H deglycosylated Pfs48/45 was recognized by conformational specific Pfs48/45 monoclonal antibody, in a manner similar to its PNGase F deglycosylated counterpart. Furthermore, the deglycosylated PA83 molecule produced by Endo H showed better stability than a PNGase F deglycosylated counterpart. Thus, an Endo H in vivo deglycosylation approach provides another opportunity to develop vaccine antigens, therapeutic proteins, antibodies, and industrial enzymes.", 
  "headline": "In vivo production of non-glycosylated recombinant proteins in Nicotiana benthamiana plants by co-expression with Endo-beta-N-acetylglucosaminidase H (Endo H) of Streptomyces plicatus", 
  "identifier": 48931, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "In vivo production of non-glycosylated recombinant proteins in Nicotiana benthamiana plants by co-expression with Endo-beta-N-acetylglucosaminidase H (Endo H) of Streptomyces plicatus", 
  "url": "https://aperta.ulakbim.gov.tr/record/48931"
}