1 Ocak 2017 Dergi makalesi Açık Erişim

Mamedov, Tarlan; Cicek, Kader; Gulec, Burcu; Ungor, Rifat; Hasanova, Gulnara

JSON

{
  "conceptrecid": "48930", 
  "created": "2021-03-15T22:22:37.200925+00:00", 
  "doi": "10.1371/journal.pone.0183589", 
  "files": [
    {
      "bucket": "dfcc606d-6f26-45df-8cdd-f31c531ebc87", 
      "checksum": "md5:6f8979811aba915943cc191d2a5a0869", 
      "key": "bib-1777cb8a-6aae-4784-bdd6-623eef62df37.txt", 
      "links": {
        "self": "https://aperta.ulakbim.gov.tr/api/files/dfcc606d-6f26-45df-8cdd-f31c531ebc87/bib-1777cb8a-6aae-4784-bdd6-623eef62df37.txt"
      }, 
      "size": 269, 
      "type": "txt"
    }
  ], 
  "id": 48931, 
  "links": {
    "badge": "https://aperta.ulakbim.gov.tr/badge/doi/10.1371/journal.pone.0183589.svg", 
    "bucket": "https://aperta.ulakbim.gov.tr/api/files/dfcc606d-6f26-45df-8cdd-f31c531ebc87", 
    "doi": "https://doi.org/10.1371/journal.pone.0183589", 
    "html": "https://aperta.ulakbim.gov.tr/record/48931", 
    "latest": "https://aperta.ulakbim.gov.tr/api/records/48931", 
    "latest_html": "https://aperta.ulakbim.gov.tr/record/48931"
  }, 
  "metadata": {
    "access_right": "open", 
    "access_right_category": "success", 
    "communities": [
      {
        "id": "tubitak-destekli-proje-yayinlari"
      }
    ], 
    "creators": [
      {
        "affiliation": "Akdeniz Univ, Dept Agr Biotechnol, Antalya, Turkey", 
        "name": "Mamedov, Tarlan"
      }, 
      {
        "affiliation": "Akdeniz Univ, Dept Agr Biotechnol, Antalya, Turkey", 
        "name": "Cicek, Kader"
      }, 
      {
        "affiliation": "Akdeniz Univ, Dept Agr Biotechnol, Antalya, Turkey", 
        "name": "Gulec, Burcu"
      }, 
      {
        "affiliation": "Akdeniz Univ, Dept Agr Biotechnol, Antalya, Turkey", 
        "name": "Ungor, Rifat"
      }, 
      {
        "affiliation": "Akdeniz Univ, Dept Agr Biotechnol, Antalya, Turkey", 
        "name": "Hasanova, Gulnara"
      }
    ], 
    "description": "A plant transient expression system, with eukaryotic post-translational modification machinery, offers superior efficiency, scalability, safety, and lower cost over other expression systems. However, due to aberrant N-glycosylation, this expression system may not be a suitable expression platform for proteins not carrying N-linked glycans in the native hosts. Therefore, it is crucial to develop a strategy to produce target proteins in a non-glycosylated form while preserving their native sequence, conformation and biological activity. Previously, we developed a strategy for enzymatic deglycosylation of proteins in planta by co-expressing bacterial peptide-N-glycosidase F (PNGase F). Though PNGase F removes oligosaccharides from glycosylated proteins, in so doing it causes an amino acid change due to the deamidation of asparagine to aspartate in the N-X-S/T site. Endo-beta-N-acetylglucosaminidase (EC3.2.1.96, Endo H), another deglycosylating enzyme, catalyzes cleavage between two N-Acetyl-D-glucosamine residues of the chitobiose core of N-linked glycans, leaving a single N-Acetyl-D-glucosamine residue without the concomitant deamidation of asparagine. In this study, a method for in vivo deglycosylation of recombinant proteins in plants by transient co-expression with bacterial Endo H is described for the first time. Endo H was fully active in vivo. and successfully cleaved N-linked glycans from glycoproteins were tested. In addition, unlike the glycosylated form, in vivo Endo H deglycosylated Pfs48/45 was recognized by conformational specific Pfs48/45 monoclonal antibody, in a manner similar to its PNGase F deglycosylated counterpart. Furthermore, the deglycosylated PA83 molecule produced by Endo H showed better stability than a PNGase F deglycosylated counterpart. Thus, an Endo H in vivo deglycosylation approach provides another opportunity to develop vaccine antigens, therapeutic proteins, antibodies, and industrial enzymes.", 
    "doi": "10.1371/journal.pone.0183589", 
    "has_grant": false, 
    "journal": {
      "issue": "8", 
      "title": "PLOS ONE", 
      "volume": "12"
    }, 
    "license": {
      "id": "cc-by"
    }, 
    "publication_date": "2017-01-01", 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "48931"
          }, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "48930"
          }
        }
      ]
    }, 
    "resource_type": {
      "subtype": "article", 
      "title": "Dergi makalesi", 
      "type": "publication"
    }, 
    "title": "In vivo production of non-glycosylated recombinant proteins in Nicotiana benthamiana plants by co-expression with Endo-beta-N-acetylglucosaminidase H (Endo H) of Streptomyces plicatus"
  }, 
  "owners": [
    1
  ], 
  "revision": 1, 
  "stats": {
    "downloads": 4.0, 
    "unique_downloads": 4.0, 
    "unique_views": 41.0, 
    "version_downloads": 4.0, 
    "version_unique_downloads": 4.0, 
    "version_unique_views": 41.0, 
    "version_views": 42.0, 
    "version_volume": 1076.0, 
    "views": 42.0, 
    "volume": 1076.0
  }, 
  "updated": "2021-03-15T22:22:37.247031+00:00"
}