1 Ocak 2024 Dergi makalesi Açık Erişim

Topcu, Beril Tas; Kaplan, Ozan; Pehlivan, Sibel Bozdag; Celebier, Mustafa; Oner, Levent

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  <dc:creator>Topcu, Beril Tas</dc:creator>
  <dc:creator>Kaplan, Ozan</dc:creator>
  <dc:creator>Pehlivan, Sibel Bozdag</dc:creator>
  <dc:creator>Celebier, Mustafa</dc:creator>
  <dc:creator>Oner, Levent</dc:creator>
  <dc:date>2024-01-01</dc:date>
  <dc:description>The use of poly (ADP -ribose) polymerase (PARP) inhibitors for cancer treatment has been reported previously. Talazoparib is a PARP inhibitor, and its solubility problems encouraged us to prepare talazoparib-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles for use in brain cancer models. To determine the encapsulation efficiency and release profile, a reversed -phase high-pressure liquid chromatography (RP-HPLC) method was developed and validated. A Shiseido 5 mu m C18 100 angstrom column (250 x 4.6 mm) was used with a flow rate of 1.0 mL/min. Isocratic elution was performed using an acetonitrile:phosphate buffer (100 mm, pH 6.25) (35:65 v/v) mixture. The injection volume was 5 mu L and UV detection was performed at 227 nm. The method was linear within the range from 0.1 to 12.5 mu g/mL. The encapsulation efficiency and release profile of the prepared formulation were analyzed using the developed RP-HPLC method, and it was found that the encapsulation efficiency was 65.17% +/- 0.50 and the release of the talazoparib was around 40% within 5 h and remained stable for 25 h. The RP-HPLC method developed in the present study can be adapted for further applications to determine talazoparib in its commercial formulations and proposed encapsulated drug delivery systems.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/278015</dc:identifier>
  <dc:identifier>oai:aperta.ulakbim.gov.tr:278015</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>JOURNAL OF CHEMICAL METROLOGY 18(1) 9</dc:source>
  <dc:title>Development of an RP-HPLC method to evaluate the basic characteristics of talazoparib-loaded PLGA nanoparticles</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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