1 Ocak 2024 Dergi makalesi Açık Erişim

Topcu, Beril Tas; Kaplan, Ozan; Pehlivan, Sibel Bozdag; Celebier, Mustafa; Oner, Levent

JSON

{
  "conceptrecid": "278014", 
  "created": "2025-04-17T16:55:14.514504+00:00", 
  "doi": "10.25135/jcm.2310.2937", 
  "files": [
    {
      "bucket": "c8d9a64b-a265-4b38-817d-3796da0cfdc7", 
      "checksum": "md5:2a16f3ca175209f8ba61f9b7c4e63913", 
      "key": "10-25135-jcm-2310-2937.pdf", 
      "links": {
        "self": "https://aperta.ulakbim.gov.tr/api/files/c8d9a64b-a265-4b38-817d-3796da0cfdc7/10-25135-jcm-2310-2937.pdf"
      }, 
      "size": 520443, 
      "type": "pdf"
    }
  ], 
  "id": 278015, 
  "links": {
    "badge": "https://aperta.ulakbim.gov.tr/badge/doi/10.25135/jcm.2310.2937.svg", 
    "bucket": "https://aperta.ulakbim.gov.tr/api/files/c8d9a64b-a265-4b38-817d-3796da0cfdc7", 
    "doi": "https://doi.org/10.25135/jcm.2310.2937", 
    "html": "https://aperta.ulakbim.gov.tr/record/278015", 
    "latest": "https://aperta.ulakbim.gov.tr/api/records/278015", 
    "latest_html": "https://aperta.ulakbim.gov.tr/record/278015"
  }, 
  "metadata": {
    "access_right": "open", 
    "access_right_category": "success", 
    "communities": [
      {
        "id": "tubitak-destekli-proje-yayinlari"
      }
    ], 
    "creators": [
      {
        "affiliation": "Hacettepe Univ, Fac Pharm, Dept Pharmaceut Technol, Ankara, Turkiye", 
        "name": "Topcu, Beril Tas"
      }, 
      {
        "affiliation": "Hacettepe Univ, Fac Pharm, Dept Analyt Chem, Ankara, Turkiye", 
        "name": "Kaplan, Ozan"
      }, 
      {
        "affiliation": "Hacettepe Univ, Fac Pharm, Dept Pharmaceut Technol, Ankara, Turkiye", 
        "name": "Pehlivan, Sibel Bozdag"
      }, 
      {
        "affiliation": "Hacettepe Univ, Fac Pharm, Dept Analyt Chem, Ankara, Turkiye", 
        "name": "Celebier, Mustafa"
      }, 
      {
        "affiliation": "Hacettepe Univ, Fac Pharm, Dept Pharmaceut Technol, Ankara, Turkiye", 
        "name": "Oner, Levent"
      }
    ], 
    "description": "<p>The use of poly (ADP -ribose) polymerase (PARP) inhibitors for cancer treatment has been reported previously. Talazoparib is a PARP inhibitor, and its solubility problems encouraged us to prepare talazoparib-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles for use in brain cancer models. To determine the encapsulation efficiency and release profile, a reversed -phase high-pressure liquid chromatography (RP-HPLC) method was developed and validated. A Shiseido 5 mu m C18 100 angstrom column (250 x 4.6 mm) was used with a flow rate of 1.0 mL/min. Isocratic elution was performed using an acetonitrile:phosphate buffer (100 mm, pH 6.25) (35:65 v/v) mixture. The injection volume was 5 mu L and UV detection was performed at 227 nm. The method was linear within the range from 0.1 to 12.5 mu g/mL. The encapsulation efficiency and release profile of the prepared formulation were analyzed using the developed RP-HPLC method, and it was found that the encapsulation efficiency was 65.17% +/- 0.50 and the release of the talazoparib was around 40% within 5 h and remained stable for 25 h. The RP-HPLC method developed in the present study can be adapted for further applications to determine talazoparib in its commercial formulations and proposed encapsulated drug delivery systems.</p>", 
    "doi": "10.25135/jcm.2310.2937", 
    "has_grant": false, 
    "journal": {
      "issue": "1", 
      "pages": "9", 
      "title": "JOURNAL OF CHEMICAL METROLOGY", 
      "volume": "18"
    }, 
    "license": {
      "id": "cc-by"
    }, 
    "publication_date": "2024-01-01", 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "278015"
          }, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "278014"
          }
        }
      ]
    }, 
    "resource_type": {
      "subtype": "article", 
      "title": "Dergi makalesi", 
      "type": "publication"
    }, 
    "science_branches": [
      "Di\u011fer"
    ], 
    "title": "Development of an RP-HPLC method to evaluate the basic characteristics of talazoparib-loaded PLGA nanoparticles"
  }, 
  "owners": [
    1
  ], 
  "revision": 1, 
  "stats": {
    "downloads": 0.0, 
    "unique_downloads": 0.0, 
    "unique_views": 0.0, 
    "version_downloads": 0.0, 
    "version_unique_downloads": 0.0, 
    "version_unique_views": 0.0, 
    "version_views": 0.0, 
    "version_volume": 0.0, 
    "views": 0.0, 
    "volume": 0.0
  }, 
  "updated": "2025-04-17T16:55:14.555301+00:00"
}