1 Ocak 2024 Dergi makalesi Açık Erişim

Topcu, Beril Tas; Kaplan, Ozan; Pehlivan, Sibel Bozdag; Celebier, Mustafa; Oner, Levent

Citation Style Language JSON

{
  "DOI": "10.25135/jcm.2310.2937", 
  "abstract": "<p>The use of poly (ADP -ribose) polymerase (PARP) inhibitors for cancer treatment has been reported previously. Talazoparib is a PARP inhibitor, and its solubility problems encouraged us to prepare talazoparib-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles for use in brain cancer models. To determine the encapsulation efficiency and release profile, a reversed -phase high-pressure liquid chromatography (RP-HPLC) method was developed and validated. A Shiseido 5 mu m C18 100 angstrom column (250 x 4.6 mm) was used with a flow rate of 1.0 mL/min. Isocratic elution was performed using an acetonitrile:phosphate buffer (100 mm, pH 6.25) (35:65 v/v) mixture. The injection volume was 5 mu L and UV detection was performed at 227 nm. The method was linear within the range from 0.1 to 12.5 mu g/mL. The encapsulation efficiency and release profile of the prepared formulation were analyzed using the developed RP-HPLC method, and it was found that the encapsulation efficiency was 65.17% +/- 0.50 and the release of the talazoparib was around 40% within 5 h and remained stable for 25 h. The RP-HPLC method developed in the present study can be adapted for further applications to determine talazoparib in its commercial formulations and proposed encapsulated drug delivery systems.</p>", 
  "author": [
    {
      "family": "Topcu", 
      "given": " Beril Tas"
    }, 
    {
      "family": "Kaplan", 
      "given": " Ozan"
    }, 
    {
      "family": "Pehlivan", 
      "given": " Sibel Bozdag"
    }, 
    {
      "family": "Celebier", 
      "given": " Mustafa"
    }, 
    {
      "family": "Oner", 
      "given": " Levent"
    }
  ], 
  "container_title": "JOURNAL OF CHEMICAL METROLOGY", 
  "id": "278015", 
  "issue": "1", 
  "issued": {
    "date-parts": [
      [
        2024, 
        1, 
        1
      ]
    ]
  }, 
  "page": "9", 
  "title": "Development of an RP-HPLC method to evaluate the basic characteristics of talazoparib-loaded PLGA nanoparticles", 
  "type": "article-journal", 
  "volume": "18"
}