1 Ocak 2023 Dergi makalesi Açık Erişim

Ozyilmaz, Elif; Caglar, Ozge

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/269034</identifier>
  <creators>
    <creator>
      <creatorName>Ozyilmaz, Elif</creatorName>
      <givenName>Elif</givenName>
      <familyName>Ozyilmaz</familyName>
      <affiliation>Selcuk Univ, Dept Biochem, TR-42075 Konya, Turkiye</affiliation>
    </creator>
    <creator>
      <creatorName>Caglar, Ozge</creatorName>
      <givenName>Ozge</givenName>
      <familyName>Caglar</familyName>
    </creator>
  </creators>
  <titles>
    <title>Rapid Mechanochemical Production Of Biocomposites By Encapsulating Enzymes To Zinc Based-Two Metal Organic Frameworks (Zif-8 And Zn-Mof-74) For Enantioselective Hydrolysis Reaction Of Racemic Naproxen Methyl Ester</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2023</publicationYear>
  <dates>
    <date dateType="Issued">2023-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/269034</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.procbio.2023.10.002</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;The mechanochemical encapsulation of enzymes during the synthesis of metal-organic frameworks (MOF) have been thought to be a natural, economical, new, and environmentally friendly step in synthesizing supported enzymatic catalysts. In this study, for the first time, a mechanochemical technique was employed to immobilize Candida rugosa lipase (CRL), and two zinc-based MOF types (Zn-MOF-74 and ZIF-8) were preferred. The enzyme kinetics and immobilization parameters (pH, temperature, thermal stability and reusability) of the prepared encapsulated enzymes (Zn-MOF-74@CRL and ZIF-8@CRL) were investigated. The prepared encapsulated enzymes were characterized by FTIR, SEM and XRD. It was observed that the catalytic activity of Zn-MOF-74 @CRL was six times higher than that of ZIF-8@CRL. After five reuses, the activities of Zn-MOF-74@CRL and ZIF-8@CRL were found to be 81% and 73% retained, respectively. After being subjected to 60 degrees C for 120 min, ZIF-8@CRL and Zn-MOF-74@CRL maintained their activities at 65% and 73%, respectively. The enantioselective hydrolysis reaction of racemic naproxen methyl ester was chosen as the model reaction. The results showed Zn-MOF-74@CRL possessed better enantioselectivity (E = 245) than ZIF-8@CRL (E = 136). This study demonstrated that it is possible to create biocatalysts with excellent catalytic properties for the pharmaceutical industry using MOFs produced by the mechanochemical method.&lt;/p&gt;</description>
  </descriptions>
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