1 Ocak 2023 Dergi makalesi Açık Erişim

Ozyilmaz, Elif; Caglar, Ozge

Citation Style Language JSON

{
  "DOI": "10.1016/j.procbio.2023.10.002", 
  "abstract": "<p>The mechanochemical encapsulation of enzymes during the synthesis of metal-organic frameworks (MOF) have been thought to be a natural, economical, new, and environmentally friendly step in synthesizing supported enzymatic catalysts. In this study, for the first time, a mechanochemical technique was employed to immobilize Candida rugosa lipase (CRL), and two zinc-based MOF types (Zn-MOF-74 and ZIF-8) were preferred. The enzyme kinetics and immobilization parameters (pH, temperature, thermal stability and reusability) of the prepared encapsulated enzymes (Zn-MOF-74@CRL and ZIF-8@CRL) were investigated. The prepared encapsulated enzymes were characterized by FTIR, SEM and XRD. It was observed that the catalytic activity of Zn-MOF-74 @CRL was six times higher than that of ZIF-8@CRL. After five reuses, the activities of Zn-MOF-74@CRL and ZIF-8@CRL were found to be 81% and 73% retained, respectively. After being subjected to 60 degrees C for 120 min, ZIF-8@CRL and Zn-MOF-74@CRL maintained their activities at 65% and 73%, respectively. The enantioselective hydrolysis reaction of racemic naproxen methyl ester was chosen as the model reaction. The results showed Zn-MOF-74@CRL possessed better enantioselectivity (E = 245) than ZIF-8@CRL (E = 136). This study demonstrated that it is possible to create biocatalysts with excellent catalytic properties for the pharmaceutical industry using MOFs produced by the mechanochemical method.</p>", 
  "author": [
    {
      "family": "Ozyilmaz", 
      "given": " Elif"
    }, 
    {
      "family": "Caglar", 
      "given": " Ozge"
    }
  ], 
  "container_title": "PROCESS BIOCHEMISTRY", 
  "id": "269034", 
  "issued": {
    "date-parts": [
      [
        2023, 
        1, 
        1
      ]
    ]
  }, 
  "page": "10", 
  "title": "Rapid mechanochemical production of biocomposites by encapsulating enzymes to zinc based-two metal organic frameworks (ZIF-8 and Zn-MOF-74) for enantioselective hydrolysis reaction of racemic Naproxen methyl ester", 
  "type": "article-journal", 
  "volume": "134"
}