1 Ocak 2023 Dergi makalesi Açık Erişim

Isil, Turp; Ozlem, Kara; Defne, Burduroglu Hatice; Eray, Guler Metin; Abdurrahim, Kocyigit

Dublin Core

<?xml version='1.0' encoding='utf-8'?>
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Isil, Turp</dc:creator>
  <dc:creator>Ozlem, Kara</dc:creator>
  <dc:creator>Defne, Burduroglu Hatice</dc:creator>
  <dc:creator>Eray, Guler Metin</dc:creator>
  <dc:creator>Abdurrahim, Kocyigit</dc:creator>
  <dc:date>2023-01-01</dc:date>
  <dc:description>Background: The aim of the study is to determine the cytotoxic, genotoxic and inflammatory effects of indocyanine green (ICG) mediated photodynamic therapy (PDT) in direct contact with L-929 mouse fibroblast cells and over a dentin barrier.Methods: Eight groups were evaluated; control (C), group with a dentin barrier (D), ICG applied directly on the cells (ICG), ICG applied over a dentin barrier (D-ICG), only laser applied (L), laser applied over a dentin barrier (D-L), ICG and laser applied directly on the cells (ICG-L), ICG and laser applied over a dentin barrier (D-ICG-L). Cell viability was evaluated via ATP Assay, DNA damage was evaluated via Comet Assay, and inflammatory markers IL-1I3 and TNF-a were assessed via ELISA test.Results: Cell viability decreased in group ICG (p&lt;0.001). Cell viability decrease was higher in Group ICG-L (p&lt;0.001). Cell viability decrease was lower in group D-ICG-L (p&gt;0.05). Group L caused an increase in cell number (p&lt;0.001). DNA damage was observed in ICG, D-ICG, and ICG-L groups (p&lt;0.05). None of the groups displayed an increase of inflammatory markers IL-1I3 and TNF-a (p&gt;0.05).Conclusions: The presence of dentin between ICG and cells acted as a barrier and protected the cells. ICGmediated PDT did not cause any cytotoxic, genotoxic or inflammatory effect. The use of ICG-mediated PDT for cavity disinfection is acceptable, but at this concentration its use in periodontal pocket disinfection is not recommended due to its cytotoxic and genotoxic properties.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/267364</dc:identifier>
  <dc:identifier>oai:aperta.ulakbim.gov.tr:267364</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY 44 8</dc:source>
  <dc:title>Toxicity evaluation of indocyanine green mediated photodynamic therapy</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
</oai_dc:dc>