1 Ocak 2023 Dergi makalesi Açık Erişim

Isil, Turp; Ozlem, Kara; Defne, Burduroglu Hatice; Eray, Guler Metin; Abdurrahim, Kocyigit

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{
  "@context": "https://schema.org/", 
  "@id": 267364, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Bezmialem Vakif Univ, Fac Dent, Dept Prosthodont, Istanbul, Turkiye", 
      "name": "Isil, Turp"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Bezmialem Vakif Univ, Fac Dent, Dept Prosthodont, Istanbul, Turkiye", 
      "name": "Ozlem, Kara"
    }, 
    {
      "@type": "Person", 
      "affiliation": "President Clin, Istanbul, Turkiye", 
      "name": "Defne, Burduroglu Hatice"
    }, 
    {
      "@type": "Person", 
      "name": "Eray, Guler Metin"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Bezmialem Vakif Univ, Fac Med, Dept Med Biochem, Istanbul, Turkiye", 
      "name": "Abdurrahim, Kocyigit"
    }
  ], 
  "datePublished": "2023-01-01", 
  "description": "<p>Background: The aim of the study is to determine the cytotoxic, genotoxic and inflammatory effects of indocyanine green (ICG) mediated photodynamic therapy (PDT) in direct contact with L-929 mouse fibroblast cells and over a dentin barrier.Methods: Eight groups were evaluated; control (C), group with a dentin barrier (D), ICG applied directly on the cells (ICG), ICG applied over a dentin barrier (D-ICG), only laser applied (L), laser applied over a dentin barrier (D-L), ICG and laser applied directly on the cells (ICG-L), ICG and laser applied over a dentin barrier (D-ICG-L). Cell viability was evaluated via ATP Assay, DNA damage was evaluated via Comet Assay, and inflammatory markers IL-1I3 and TNF-a were assessed via ELISA test.Results: Cell viability decreased in group ICG (p&lt;0.001). Cell viability decrease was higher in Group ICG-L (p&lt;0.001). Cell viability decrease was lower in group D-ICG-L (p&gt;0.05). Group L caused an increase in cell number (p&lt;0.001). DNA damage was observed in ICG, D-ICG, and ICG-L groups (p&lt;0.05). None of the groups displayed an increase of inflammatory markers IL-1I3 and TNF-a (p&gt;0.05).Conclusions: The presence of dentin between ICG and cells acted as a barrier and protected the cells. ICGmediated PDT did not cause any cytotoxic, genotoxic or inflammatory effect. The use of ICG-mediated PDT for cavity disinfection is acceptable, but at this concentration its use in periodontal pocket disinfection is not recommended due to its cytotoxic and genotoxic properties.</p>", 
  "headline": "Toxicity evaluation of indocyanine green mediated photodynamic therapy", 
  "identifier": 267364, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Toxicity evaluation of indocyanine green mediated photodynamic therapy", 
  "url": "https://aperta.ulakbim.gov.tr/record/267364"
}