Dergi makalesi Açık Erişim
Aksoy, Secil Ak; Tunca, Berrin; Ercelik, Melis; Tezcan, Gulcin; Ozturk, Ersin; Cecener, Gulsah; Ugras, Nesrin; Yilmazlar, Tuncay; Yerci, Omer
{ "@context": "https://schema.org/", "@id": 258999, "@type": "ScholarlyArticle", "creator": [ { "@type": "Person", "affiliation": "Uludag Univ, Vocat Sch Inegol, Bursa, Turkey", "name": "Aksoy, Secil Ak" }, { "@type": "Person", "affiliation": "Uludag Univ, Med Fac, Dept Med Biol, Bursa, Turkey", "name": "Tunca, Berrin" }, { "@type": "Person", "affiliation": "Uludag Univ, Med Fac, Dept Med Biol, Bursa, Turkey", "name": "Ercelik, Melis" }, { "@type": "Person", "affiliation": "Bursa Uludag Univ, Dept Fundamental Sci, Fac Dent, Bursa, Turkey", "name": "Tezcan, Gulcin" }, { "@type": "Person", "affiliation": "Turkey Gen Surg, Dept Gen Surg, Med, Bursa, Turkey", "name": "Ozturk, Ersin" }, { "@type": "Person", "affiliation": "Uludag Univ, Med Fac, Dept Med Biol, Bursa, Turkey", "name": "Cecener, Gulsah" }, { "@type": "Person", "affiliation": "Uludag Univ, Med Fac, Dept Pathol, Bursa, Turkey", "name": "Ugras, Nesrin" }, { "@type": "Person", "affiliation": "Uludag Univ, Med Fac, Dept Gen Surg, Bursa, Turkey", "name": "Yilmazlar, Tuncay" }, { "@type": "Person", "affiliation": "Uludag Univ, Med Fac, Dept Pathol, Bursa, Turkey", "name": "Yerci, Omer" } ], "datePublished": "2022-01-01", "description": "Background This study aimed to investigate the role of long noncoding RNA (LncRNA) expression profiles to predict relapse and 5-FU response in patients with stage I/II colon cancer (CC). Methods and Results The expression level of 15 LncRNA was analyzed in stage I/II colon tumors of 126 CC patients. To confirm the findings in-vitro, 5FU-resistant HT29 cells were generated by subjecting HT-29 cells to the increasing concentrations of 5FU for 6 months. The 5FU resistance was observed in WST-1 and Annexin V analyses. The colony formation and wound healing assays were assessed to determine the metastatic properties of the cells. Expression levels of LncRNAs and mRNA of EMT-related genes were determined by RT-PCR. The role of LncRNA on metastasis and 5FU sensitivity were confirmed in pcDNA3.0-PTENP1 and si-MALAT1 expressed 5FU-resistant HT29 cell lineages. Results High MALAT1 (p = 0.0002) and low PTENP1 (p = 0.0044) expressions were significantly associated with 5-FU resistance and tumor relapse in stage I/II CC. The invasiveness and colony-forming characteristics of 5-FU-resistant cell lineages were higher as compared to the parent HT-29. Moreover, the expression of MALAT1 (p = 0.0009) was increased while the expression of PTENP1 (p = 0.0158) decreased in 5FU-resistant-HT-29 cells. Si-MALAT1 treatment increased cell sensitivity to 5FU, whereas it decreased invasive behaviors of 5 FU-resistant-HT-29 cells. Conclusion MALAT1 may be a biomarker in predicting recurrence in early-stage CC. Our findings suggest that a cell-based therapy to target MALAT1 could be established for these patients to prevent metastasis and 5-FU resistance.", "headline": "Early-stage colon cancer with high MALAT1 expression is associated with the 5-Fluorouracil resistance and future metastasis", "identifier": 258999, "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", "license": "http://www.opendefinition.org/licenses/cc-by", "name": "Early-stage colon cancer with high MALAT1 expression is associated with the 5-Fluorouracil resistance and future metastasis", "url": "https://aperta.ulakbim.gov.tr/record/258999" }
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