1 Ocak 2022 Dergi makalesi Açık Erişim

Aksoy, Secil Ak; Tunca, Berrin; Ercelik, Melis; Tezcan, Gulcin; Ozturk, Ersin; Cecener, Gulsah; Ugras, Nesrin; Yilmazlar, Tuncay; Yerci, Omer

Citation Style Language JSON

{
  "DOI": "10.1007/s11033-022-07680-y", 
  "abstract": "Background This study aimed to investigate the role of long noncoding RNA (LncRNA) expression profiles to predict relapse and 5-FU response in patients with stage I/II colon cancer (CC). Methods and Results The expression level of 15 LncRNA was analyzed in stage I/II colon tumors of 126 CC patients. To confirm the findings in-vitro, 5FU-resistant HT29 cells were generated by subjecting HT-29 cells to the increasing concentrations of 5FU for 6 months. The 5FU resistance was observed in WST-1 and Annexin V analyses. The colony formation and wound healing assays were assessed to determine the metastatic properties of the cells. Expression levels of LncRNAs and mRNA of EMT-related genes were determined by RT-PCR. The role of LncRNA on metastasis and 5FU sensitivity were confirmed in pcDNA3.0-PTENP1 and si-MALAT1 expressed 5FU-resistant HT29 cell lineages. Results High MALAT1 (p = 0.0002) and low PTENP1 (p = 0.0044) expressions were significantly associated with 5-FU resistance and tumor relapse in stage I/II CC. The invasiveness and colony-forming characteristics of 5-FU-resistant cell lineages were higher as compared to the parent HT-29. Moreover, the expression of MALAT1 (p = 0.0009) was increased while the expression of PTENP1 (p = 0.0158) decreased in 5FU-resistant-HT-29 cells. Si-MALAT1 treatment increased cell sensitivity to 5FU, whereas it decreased invasive behaviors of 5 FU-resistant-HT-29 cells. Conclusion MALAT1 may be a biomarker in predicting recurrence in early-stage CC. Our findings suggest that a cell-based therapy to target MALAT1 could be established for these patients to prevent metastasis and 5-FU resistance.", 
  "author": [
    {
      "family": "Aksoy", 
      "given": " Secil Ak"
    }, 
    {
      "family": "Tunca", 
      "given": " Berrin"
    }, 
    {
      "family": "Ercelik", 
      "given": " Melis"
    }, 
    {
      "family": "Tezcan", 
      "given": " Gulcin"
    }, 
    {
      "family": "Ozturk", 
      "given": " Ersin"
    }, 
    {
      "family": "Cecener", 
      "given": " Gulsah"
    }, 
    {
      "family": "Ugras", 
      "given": " Nesrin"
    }, 
    {
      "family": "Yilmazlar", 
      "given": " Tuncay"
    }, 
    {
      "family": "Yerci", 
      "given": " Omer"
    }
  ], 
  "container_title": "MOLECULAR BIOLOGY REPORTS", 
  "id": "258999", 
  "issue": "12", 
  "issued": {
    "date-parts": [
      [
        2022, 
        1, 
        1
      ]
    ]
  }, 
  "page": "11243-11253", 
  "title": "Early-stage colon cancer with high MALAT1 expression is associated with the 5-Fluorouracil resistance and future metastasis", 
  "type": "article-journal", 
  "volume": "49"
}