1 Ocak 2022 Dergi makalesi Açık Erişim

Aksoy, Secil Ak; Tunca, Berrin; Ercelik, Melis; Tezcan, Gulcin; Ozturk, Ersin; Cecener, Gulsah; Ugras, Nesrin; Yilmazlar, Tuncay; Yerci, Omer

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  "created": "2023-07-29T08:26:14.591301+00:00", 
  "doi": "10.1007/s11033-022-07680-y", 
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    "communities": [
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        "id": "tubitak-destekli-proje-yayinlari"
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    "creators": [
      {
        "affiliation": "Uludag Univ, Vocat Sch Inegol, Bursa, Turkey", 
        "name": "Aksoy, Secil Ak"
      }, 
      {
        "affiliation": "Uludag Univ, Med Fac, Dept Med Biol, Bursa, Turkey", 
        "name": "Tunca, Berrin"
      }, 
      {
        "affiliation": "Uludag Univ, Med Fac, Dept Med Biol, Bursa, Turkey", 
        "name": "Ercelik, Melis"
      }, 
      {
        "affiliation": "Bursa Uludag Univ, Dept Fundamental Sci, Fac Dent, Bursa, Turkey", 
        "name": "Tezcan, Gulcin"
      }, 
      {
        "affiliation": "Turkey Gen Surg, Dept Gen Surg, Med, Bursa, Turkey", 
        "name": "Ozturk, Ersin"
      }, 
      {
        "affiliation": "Uludag Univ, Med Fac, Dept Med Biol, Bursa, Turkey", 
        "name": "Cecener, Gulsah"
      }, 
      {
        "affiliation": "Uludag Univ, Med Fac, Dept Pathol, Bursa, Turkey", 
        "name": "Ugras, Nesrin"
      }, 
      {
        "affiliation": "Uludag Univ, Med Fac, Dept Gen Surg, Bursa, Turkey", 
        "name": "Yilmazlar, Tuncay"
      }, 
      {
        "affiliation": "Uludag Univ, Med Fac, Dept Pathol, Bursa, Turkey", 
        "name": "Yerci, Omer"
      }
    ], 
    "description": "Background This study aimed to investigate the role of long noncoding RNA (LncRNA) expression profiles to predict relapse and 5-FU response in patients with stage I/II colon cancer (CC). Methods and Results The expression level of 15 LncRNA was analyzed in stage I/II colon tumors of 126 CC patients. To confirm the findings in-vitro, 5FU-resistant HT29 cells were generated by subjecting HT-29 cells to the increasing concentrations of 5FU for 6 months. The 5FU resistance was observed in WST-1 and Annexin V analyses. The colony formation and wound healing assays were assessed to determine the metastatic properties of the cells. Expression levels of LncRNAs and mRNA of EMT-related genes were determined by RT-PCR. The role of LncRNA on metastasis and 5FU sensitivity were confirmed in pcDNA3.0-PTENP1 and si-MALAT1 expressed 5FU-resistant HT29 cell lineages. Results High MALAT1 (p = 0.0002) and low PTENP1 (p = 0.0044) expressions were significantly associated with 5-FU resistance and tumor relapse in stage I/II CC. The invasiveness and colony-forming characteristics of 5-FU-resistant cell lineages were higher as compared to the parent HT-29. Moreover, the expression of MALAT1 (p = 0.0009) was increased while the expression of PTENP1 (p = 0.0158) decreased in 5FU-resistant-HT-29 cells. Si-MALAT1 treatment increased cell sensitivity to 5FU, whereas it decreased invasive behaviors of 5 FU-resistant-HT-29 cells. Conclusion MALAT1 may be a biomarker in predicting recurrence in early-stage CC. Our findings suggest that a cell-based therapy to target MALAT1 could be established for these patients to prevent metastasis and 5-FU resistance.", 
    "doi": "10.1007/s11033-022-07680-y", 
    "has_grant": false, 
    "journal": {
      "issue": "12", 
      "pages": "11243-11253", 
      "title": "MOLECULAR BIOLOGY REPORTS", 
      "volume": "49"
    }, 
    "license": {
      "id": "cc-by"
    }, 
    "publication_date": "2022-01-01", 
    "relations": {
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    "resource_type": {
      "subtype": "article", 
      "title": "Dergi makalesi", 
      "type": "publication"
    }, 
    "science_branches": [
      "Di\u011fer"
    ], 
    "title": "Early-stage colon cancer with high MALAT1 expression is associated with the 5-Fluorouracil resistance and future metastasis"
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