Dergi makalesi Açık Erişim

Methionine restriction and cancer treatment: a systems biology study of yeast to investigate the possible key players

   Esra Börklü

Background/Aim: Dietary restriction, mainly carbon and/or methionine restriction are among the upcoming supporting interventions along with chemotherapy in various cancers. Although dietary restriction has been proven to be beneficial, main cellular machineries affected by its administration lacks deeper information considerably, a notable pitfall on its use as a personalized nutritional approach.

Materials and methods: In this study, cellular effects of methionine restriction on a yeast model are explored via systems biology approaches. The methionine biosynthesis network, constructed by integrating interaction data with gene ontology terms, was analyzed topologically, and proved to be informative about the intertwined relationship of methionine biosynthesis and cancer. Experimentally, effects of methionine restriction on the yeast model were explored in vivo, with transcriptome analyses.

Results: The integrative analysis of the transcriptional data together with the reconstructed network gave insight into cellular machineries such as TOR, MAPK and sphingolipid-mediated signaling cascades as the mostly responsive cellular pathways in the methionine-restricted cases with Sch9p (functional orthologue of mammalian S6 kinase) being placed at the intersection of these signaling routes.

Dosyalar (57.1 kB)
Dosya adı Boyutu
Supp Table 1.xlsx
md5:83711cce514b7e836397328b3bd56640
57.1 kB İndir
295
12
görüntülenme
indirilme
Tüm sürümler Bu sürüm
Görüntülenme 295295
İndirme 1212
Veri hacmi 684.7 kB684.7 kB
Tekil görüntülenme 235235
Tekil indirme 1212

Alıntı yap