1 Ocak 2022 Dergi makalesi Açık Erişim

Cagnan, Ilgin; Keles, Mustafa; Keskus, Ayse Gokce; Tombaz, Melike; Sahan, Ozge Burcu; Aerts-Kaya, Fatima; Uckan-Cetinkaya, Duygu; Konu, Ozlen; Gunel-Ozcan, Aysen

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{
  "@context": "https://schema.org/", 
  "@id": 238442, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Cagnan, Ilgin"
    }, 
    {
      "@type": "Person", 
      "name": "Keles, Mustafa"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Bilkent Univ, Interdisciplinary Neurosci Program, Ankara, Turkey", 
      "name": "Keskus, Ayse Gokce"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Bilkent Univ, Dept Mol Biol & Genet, Ankara, Turkey", 
      "name": "Tombaz, Melike"
    }, 
    {
      "@type": "Person", 
      "name": "Sahan, Ozge Burcu"
    }, 
    {
      "@type": "Person", 
      "name": "Aerts-Kaya, Fatima"
    }, 
    {
      "@type": "Person", 
      "name": "Uckan-Cetinkaya, Duygu"
    }, 
    {
      "@type": "Person", 
      "name": "Konu, Ozlen"
    }, 
    {
      "@type": "Person", 
      "name": "Gunel-Ozcan, Aysen"
    }
  ], 
  "datePublished": "2022-01-01", 
  "description": "Fanconi anemia (FA) is a rare genetic disorder characterized by genomic instability, developmental defects, and bone marrow (BM) failure. Hematopoietic stem cells (HSCs) in BM interact with the mesenchymal stem/stromal cells (MSCs); and this partly sustains the tissue homeostasis. MicroRNAs (miRNAs) can play a critical role during these interactions possibly via paracrine mechanisms. This is the first study addressing the miRNA profile of FA BM-MSCs obtained before and after BM transplantation (preBMT and postBMT, respectively). Non-coding RNA expression profiling and quality control analyses were performed in Donors (n = 13), FA preBMT (n = 11), and FA postBMT (n = 6) BM-MSCs using GeneChip miRNA 2.0 Array. Six Donor-FA preBMT pairs were used to identify a differentially expressed miRNA expression signature containing 50 miRNAs, which exhibited a strong correlation with the signature obtained from unpaired samples. Five miRNAs (hsa-miR-146a-5p, hsa-miR-148b-3p, hsa-miR-187-3p, hsa-miR-196b-5p, and hsa-miR-25-3p) significantly downregulated in both the paired and unpaired analyses were used to generate the BM-MSCs' miRNA-BM mononuclear mRNA networks upon integration of a public dataset (GSE16334; studying Donor versus FA samples). Functionally enriched KEGG pathways included cellular senescence, miRNAs, and pathways in cancer. Here, we showed that hsa-miR-146a-5p and hsa-miR-874-3p were rescued upon BMT (n = 3 triplets). The decrease in miR-146a-5p was also validated using RT-qPCR and emerged as a strong candidate as a modulator of BM mRNAs in FA patients.", 
  "headline": "Global miRNA expression of bone marrow mesenchymal stem/stromal cells derived from Fanconi anemia patients", 
  "identifier": 238442, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Global miRNA expression of bone marrow mesenchymal stem/stromal cells derived from Fanconi anemia patients", 
  "url": "https://aperta.ulakbim.gov.tr/record/238442"
}