1 Ocak 2022 Dergi makalesi Açık Erişim

Cagnan, Ilgin; Keles, Mustafa; Keskus, Ayse Gokce; Tombaz, Melike; Sahan, Ozge Burcu; Aerts-Kaya, Fatima; Uckan-Cetinkaya, Duygu; Konu, Ozlen; Gunel-Ozcan, Aysen

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  "doi": "10.1007/s13577-021-00626-9", 
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        "id": "tubitak-destekli-proje-yayinlari"
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    "creators": [
      {
        "name": "Cagnan, Ilgin"
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      {
        "name": "Keles, Mustafa"
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      {
        "affiliation": "Bilkent Univ, Interdisciplinary Neurosci Program, Ankara, Turkey", 
        "name": "Keskus, Ayse Gokce"
      }, 
      {
        "affiliation": "Bilkent Univ, Dept Mol Biol & Genet, Ankara, Turkey", 
        "name": "Tombaz, Melike"
      }, 
      {
        "name": "Sahan, Ozge Burcu"
      }, 
      {
        "name": "Aerts-Kaya, Fatima"
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      {
        "name": "Uckan-Cetinkaya, Duygu"
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      {
        "name": "Konu, Ozlen"
      }, 
      {
        "name": "Gunel-Ozcan, Aysen"
      }
    ], 
    "description": "Fanconi anemia (FA) is a rare genetic disorder characterized by genomic instability, developmental defects, and bone marrow (BM) failure. Hematopoietic stem cells (HSCs) in BM interact with the mesenchymal stem/stromal cells (MSCs); and this partly sustains the tissue homeostasis. MicroRNAs (miRNAs) can play a critical role during these interactions possibly via paracrine mechanisms. This is the first study addressing the miRNA profile of FA BM-MSCs obtained before and after BM transplantation (preBMT and postBMT, respectively). Non-coding RNA expression profiling and quality control analyses were performed in Donors (n = 13), FA preBMT (n = 11), and FA postBMT (n = 6) BM-MSCs using GeneChip miRNA 2.0 Array. Six Donor-FA preBMT pairs were used to identify a differentially expressed miRNA expression signature containing 50 miRNAs, which exhibited a strong correlation with the signature obtained from unpaired samples. Five miRNAs (hsa-miR-146a-5p, hsa-miR-148b-3p, hsa-miR-187-3p, hsa-miR-196b-5p, and hsa-miR-25-3p) significantly downregulated in both the paired and unpaired analyses were used to generate the BM-MSCs' miRNA-BM mononuclear mRNA networks upon integration of a public dataset (GSE16334; studying Donor versus FA samples). Functionally enriched KEGG pathways included cellular senescence, miRNAs, and pathways in cancer. Here, we showed that hsa-miR-146a-5p and hsa-miR-874-3p were rescued upon BMT (n = 3 triplets). The decrease in miR-146a-5p was also validated using RT-qPCR and emerged as a strong candidate as a modulator of BM mRNAs in FA patients.", 
    "doi": "10.1007/s13577-021-00626-9", 
    "has_grant": false, 
    "journal": {
      "issue": "1", 
      "pages": "111-124", 
      "title": "HUMAN CELL", 
      "volume": "35"
    }, 
    "license": {
      "id": "cc-by"
    }, 
    "publication_date": "2022-01-01", 
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    "resource_type": {
      "subtype": "article", 
      "title": "Dergi makalesi", 
      "type": "publication"
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    "science_branches": [
      "Di\u011fer"
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    "title": "Global miRNA expression of bone marrow mesenchymal stem/stromal cells derived from Fanconi anemia patients"
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