Dergi makalesi Açık Erişim
Cagnan, Ilgin; Keles, Mustafa; Keskus, Ayse Gokce; Tombaz, Melike; Sahan, Ozge Burcu; Aerts-Kaya, Fatima; Uckan-Cetinkaya, Duygu; Konu, Ozlen; Gunel-Ozcan, Aysen
{ "conceptrecid": "238441", "created": "2022-10-07T10:13:59.502743+00:00", "doi": "10.1007/s13577-021-00626-9", "files": [ { "bucket": "ec7821b6-53af-49ba-91cd-eb39ace769eb", "checksum": "md5:a872144a8387cbc88092e1806fe94fcb", "key": "bib-622cd4fd-8e9f-4588-bae1-cd8cb06ecbb4.txt", "links": { "self": "https://aperta.ulakbim.gov.tr/api/files/ec7821b6-53af-49ba-91cd-eb39ace769eb/bib-622cd4fd-8e9f-4588-bae1-cd8cb06ecbb4.txt" }, "size": 265, "type": "txt" } ], "id": 238442, "links": { "badge": "https://aperta.ulakbim.gov.tr/badge/doi/10.1007/s13577-021-00626-9.svg", "bucket": "https://aperta.ulakbim.gov.tr/api/files/ec7821b6-53af-49ba-91cd-eb39ace769eb", "doi": "https://doi.org/10.1007/s13577-021-00626-9", "html": "https://aperta.ulakbim.gov.tr/record/238442", "latest": "https://aperta.ulakbim.gov.tr/api/records/238442", "latest_html": "https://aperta.ulakbim.gov.tr/record/238442" }, "metadata": { "access_right": "open", "access_right_category": "success", "communities": [ { "id": "tubitak-destekli-proje-yayinlari" } ], "creators": [ { "name": "Cagnan, Ilgin" }, { "name": "Keles, Mustafa" }, { "affiliation": "Bilkent Univ, Interdisciplinary Neurosci Program, Ankara, Turkey", "name": "Keskus, Ayse Gokce" }, { "affiliation": "Bilkent Univ, Dept Mol Biol & Genet, Ankara, Turkey", "name": "Tombaz, Melike" }, { "name": "Sahan, Ozge Burcu" }, { "name": "Aerts-Kaya, Fatima" }, { "name": "Uckan-Cetinkaya, Duygu" }, { "name": "Konu, Ozlen" }, { "name": "Gunel-Ozcan, Aysen" } ], "description": "Fanconi anemia (FA) is a rare genetic disorder characterized by genomic instability, developmental defects, and bone marrow (BM) failure. Hematopoietic stem cells (HSCs) in BM interact with the mesenchymal stem/stromal cells (MSCs); and this partly sustains the tissue homeostasis. MicroRNAs (miRNAs) can play a critical role during these interactions possibly via paracrine mechanisms. This is the first study addressing the miRNA profile of FA BM-MSCs obtained before and after BM transplantation (preBMT and postBMT, respectively). Non-coding RNA expression profiling and quality control analyses were performed in Donors (n = 13), FA preBMT (n = 11), and FA postBMT (n = 6) BM-MSCs using GeneChip miRNA 2.0 Array. Six Donor-FA preBMT pairs were used to identify a differentially expressed miRNA expression signature containing 50 miRNAs, which exhibited a strong correlation with the signature obtained from unpaired samples. Five miRNAs (hsa-miR-146a-5p, hsa-miR-148b-3p, hsa-miR-187-3p, hsa-miR-196b-5p, and hsa-miR-25-3p) significantly downregulated in both the paired and unpaired analyses were used to generate the BM-MSCs' miRNA-BM mononuclear mRNA networks upon integration of a public dataset (GSE16334; studying Donor versus FA samples). Functionally enriched KEGG pathways included cellular senescence, miRNAs, and pathways in cancer. Here, we showed that hsa-miR-146a-5p and hsa-miR-874-3p were rescued upon BMT (n = 3 triplets). The decrease in miR-146a-5p was also validated using RT-qPCR and emerged as a strong candidate as a modulator of BM mRNAs in FA patients.", "doi": "10.1007/s13577-021-00626-9", "has_grant": false, "journal": { "issue": "1", "pages": "111-124", "title": "HUMAN CELL", "volume": "35" }, "license": { "id": "cc-by" }, "publication_date": "2022-01-01", "relations": { "version": [ { "count": 1, "index": 0, "is_last": true, "last_child": { "pid_type": "recid", "pid_value": "238442" }, "parent": { "pid_type": "recid", "pid_value": "238441" } } ] }, "resource_type": { "subtype": "article", "title": "Dergi makalesi", "type": "publication" }, "science_branches": [ "Di\u011fer" ], "title": "Global miRNA expression of bone marrow mesenchymal stem/stromal cells derived from Fanconi anemia patients" }, "owners": [ 1 ], "revision": 1, "stats": { "downloads": 9.0, "unique_downloads": 9.0, "unique_views": 23.0, "version_downloads": 9.0, "version_unique_downloads": 9.0, "version_unique_views": 23.0, "version_views": 24.0, "version_volume": 2385.0, "views": 24.0, "volume": 2385.0 }, "updated": "2022-10-07T10:13:59.581314+00:00" }
Görüntülenme | 24 |
İndirme | 9 |
Veri hacmi | 2.4 kB |
Tekil görüntülenme | 23 |
Tekil indirme | 9 |