1 Ocak 2022 Dergi makalesi Açık Erişim

Cagnan, Ilgin; Keles, Mustafa; Keskus, Ayse Gokce; Tombaz, Melike; Sahan, Ozge Burcu; Aerts-Kaya, Fatima; Uckan-Cetinkaya, Duygu; Konu, Ozlen; Gunel-Ozcan, Aysen

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/238442</identifier>
  <creators>
    <creator>
      <creatorName>Cagnan, Ilgin</creatorName>
      <givenName>Ilgin</givenName>
      <familyName>Cagnan</familyName>
    </creator>
    <creator>
      <creatorName>Keles, Mustafa</creatorName>
      <givenName>Mustafa</givenName>
      <familyName>Keles</familyName>
    </creator>
    <creator>
      <creatorName>Keskus, Ayse Gokce</creatorName>
      <givenName>Ayse Gokce</givenName>
      <familyName>Keskus</familyName>
      <affiliation>Bilkent Univ, Interdisciplinary Neurosci Program, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Tombaz, Melike</creatorName>
      <givenName>Melike</givenName>
      <familyName>Tombaz</familyName>
      <affiliation>Bilkent Univ, Dept Mol Biol &amp; Genet, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Sahan, Ozge Burcu</creatorName>
      <givenName>Ozge Burcu</givenName>
      <familyName>Sahan</familyName>
    </creator>
    <creator>
      <creatorName>Aerts-Kaya, Fatima</creatorName>
      <givenName>Fatima</givenName>
      <familyName>Aerts-Kaya</familyName>
    </creator>
    <creator>
      <creatorName>Uckan-Cetinkaya, Duygu</creatorName>
      <givenName>Duygu</givenName>
      <familyName>Uckan-Cetinkaya</familyName>
    </creator>
    <creator>
      <creatorName>Konu, Ozlen</creatorName>
      <givenName>Ozlen</givenName>
      <familyName>Konu</familyName>
    </creator>
    <creator>
      <creatorName>Gunel-Ozcan, Aysen</creatorName>
      <givenName>Aysen</givenName>
      <familyName>Gunel-Ozcan</familyName>
    </creator>
  </creators>
  <titles>
    <title>Global Mirna Expression Of Bone Marrow Mesenchymal Stem/Stromal Cells Derived From Fanconi Anemia Patients</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2022</publicationYear>
  <dates>
    <date dateType="Issued">2022-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/238442</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1007/s13577-021-00626-9</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Fanconi anemia (FA) is a rare genetic disorder characterized by genomic instability, developmental defects, and bone marrow (BM) failure. Hematopoietic stem cells (HSCs) in BM interact with the mesenchymal stem/stromal cells (MSCs); and this partly sustains the tissue homeostasis. MicroRNAs (miRNAs) can play a critical role during these interactions possibly via paracrine mechanisms. This is the first study addressing the miRNA profile of FA BM-MSCs obtained before and after BM transplantation (preBMT and postBMT, respectively). Non-coding RNA expression profiling and quality control analyses were performed in Donors (n = 13), FA preBMT (n = 11), and FA postBMT (n = 6) BM-MSCs using GeneChip miRNA 2.0 Array. Six Donor-FA preBMT pairs were used to identify a differentially expressed miRNA expression signature containing 50 miRNAs, which exhibited a strong correlation with the signature obtained from unpaired samples. Five miRNAs (hsa-miR-146a-5p, hsa-miR-148b-3p, hsa-miR-187-3p, hsa-miR-196b-5p, and hsa-miR-25-3p) significantly downregulated in both the paired and unpaired analyses were used to generate the BM-MSCs' miRNA-BM mononuclear mRNA networks upon integration of a public dataset (GSE16334; studying Donor versus FA samples). Functionally enriched KEGG pathways included cellular senescence, miRNAs, and pathways in cancer. Here, we showed that hsa-miR-146a-5p and hsa-miR-874-3p were rescued upon BMT (n = 3 triplets). The decrease in miR-146a-5p was also validated using RT-qPCR and emerged as a strong candidate as a modulator of BM mRNAs in FA patients.</description>
  </descriptions>
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