1 Ocak 2021 Dergi makalesi Açık Erişim

Shahraki, Aida; Isbilir, Ali; Dogan, Berna; Lohse, Martin J.; Durdagi, Serdar; Birgul-Iyison, Necla

Dublin Core

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  <dc:creator>Shahraki, Aida</dc:creator>
  <dc:creator>Isbilir, Ali</dc:creator>
  <dc:creator>Dogan, Berna</dc:creator>
  <dc:creator>Lohse, Martin J.</dc:creator>
  <dc:creator>Durdagi, Serdar</dc:creator>
  <dc:creator>Birgul-Iyison, Necla</dc:creator>
  <dc:date>2021-01-01</dc:date>
  <dc:description>Insect neuropeptide receptors, including allatostatin receptor type C (AstR-C), a G protein-coupled receptor, are among the potential targets for designing next-generation pesticides that despite their importance in offering a new mode-of-action have been overlooked. Focusing on AstR-C of Thaumetopoea pityocampa, a common pest in Mediterranean countries, by employing resonance energy transfer-based methods, we showed G alpha i/o coupling and beta-arrestin recruitment of the receptor at sub-nanomolar and nanomolar ranges of the endogenous ligand, AST-C, respectively. Molecular docking and molecular dynamics simulation studies revealed the importance of extracellular loop 2 in AstRC/AST-C interaction, and a combination of in silico and in vitro approaches showed the substantial role of Q271(6.55) in G protein-dependent activation of AstR-C possibly via contributing to the flexibility of the receptor's structure. The functional and structural insights obtained on T. pit AstR-C positively assist future efforts in developing environmentally friendly pest control agents that are needed urgently.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/238024</dc:identifier>
  <dc:identifier>oai:aperta.ulakbim.gov.tr:238024</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>JOURNAL OF CHEMICAL INFORMATION AND MODELING 61(2) 715-728</dc:source>
  <dc:title>Structural and Functional Characterization of Allatostatin Receptor Type-C of Thaumetopoea pityocampa, a Potential Target for Next-Generation Pest Control Agents</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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