1 Ocak 2021 Dergi makalesi Açık Erişim

Shahraki, Aida; Isbilir, Ali; Dogan, Berna; Lohse, Martin J.; Durdagi, Serdar; Birgul-Iyison, Necla

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/238024</identifier>
  <creators>
    <creator>
      <creatorName>Shahraki, Aida</creatorName>
      <givenName>Aida</givenName>
      <familyName>Shahraki</familyName>
    </creator>
    <creator>
      <creatorName>Isbilir, Ali</creatorName>
      <givenName>Ali</givenName>
      <familyName>Isbilir</familyName>
    </creator>
    <creator>
      <creatorName>Dogan, Berna</creatorName>
      <givenName>Berna</givenName>
      <familyName>Dogan</familyName>
      <affiliation>Bahcesehir Univ, Sch Med, Dept Biophys, Computat Biol &amp; Mol Simulat Lab, TR-34734 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Lohse, Martin J.</creatorName>
      <givenName>Martin J.</givenName>
      <familyName>Lohse</familyName>
    </creator>
    <creator>
      <creatorName>Durdagi, Serdar</creatorName>
      <givenName>Serdar</givenName>
      <familyName>Durdagi</familyName>
      <affiliation>Bahcesehir Univ, Sch Med, Dept Biophys, Computat Biol &amp; Mol Simulat Lab, TR-34734 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Birgul-Iyison, Necla</creatorName>
      <givenName>Necla</givenName>
      <familyName>Birgul-Iyison</familyName>
      <affiliation>Bogazici Univ, Dept Mol Biol &amp; Genet, TR-34342 Istanbul, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Structural And Functional Characterization Of Allatostatin Receptor Type-C Of Thaumetopoea Pityocampa, A Potential Target For Next-Generation Pest Control Agents</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/238024</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1021/acs.jcim.0c00985</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Insect neuropeptide receptors, including allatostatin receptor type C (AstR-C), a G protein-coupled receptor, are among the potential targets for designing next-generation pesticides that despite their importance in offering a new mode-of-action have been overlooked. Focusing on AstR-C of Thaumetopoea pityocampa, a common pest in Mediterranean countries, by employing resonance energy transfer-based methods, we showed G alpha i/o coupling and beta-arrestin recruitment of the receptor at sub-nanomolar and nanomolar ranges of the endogenous ligand, AST-C, respectively. Molecular docking and molecular dynamics simulation studies revealed the importance of extracellular loop 2 in AstRC/AST-C interaction, and a combination of in silico and in vitro approaches showed the substantial role of Q271(6.55) in G protein-dependent activation of AstR-C possibly via contributing to the flexibility of the receptor's structure. The functional and structural insights obtained on T. pit AstR-C positively assist future efforts in developing environmentally friendly pest control agents that are needed urgently.</description>
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