1 Ocak 2021 Dergi makalesi Açık Erişim

Shahraki, Aida; Isbilir, Ali; Dogan, Berna; Lohse, Martin J.; Durdagi, Serdar; Birgul-Iyison, Necla

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{
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  "@id": 238024, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Shahraki, Aida"
    }, 
    {
      "@type": "Person", 
      "name": "Isbilir, Ali"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Bahcesehir Univ, Sch Med, Dept Biophys, Computat Biol & Mol Simulat Lab, TR-34734 Istanbul, Turkey", 
      "name": "Dogan, Berna"
    }, 
    {
      "@type": "Person", 
      "name": "Lohse, Martin J."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Bahcesehir Univ, Sch Med, Dept Biophys, Computat Biol & Mol Simulat Lab, TR-34734 Istanbul, Turkey", 
      "name": "Durdagi, Serdar"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Bogazici Univ, Dept Mol Biol & Genet, TR-34342 Istanbul, Turkey", 
      "name": "Birgul-Iyison, Necla"
    }
  ], 
  "datePublished": "2021-01-01", 
  "description": "Insect neuropeptide receptors, including allatostatin receptor type C (AstR-C), a G protein-coupled receptor, are among the potential targets for designing next-generation pesticides that despite their importance in offering a new mode-of-action have been overlooked. Focusing on AstR-C of Thaumetopoea pityocampa, a common pest in Mediterranean countries, by employing resonance energy transfer-based methods, we showed G alpha i/o coupling and beta-arrestin recruitment of the receptor at sub-nanomolar and nanomolar ranges of the endogenous ligand, AST-C, respectively. Molecular docking and molecular dynamics simulation studies revealed the importance of extracellular loop 2 in AstRC/AST-C interaction, and a combination of in silico and in vitro approaches showed the substantial role of Q271(6.55) in G protein-dependent activation of AstR-C possibly via contributing to the flexibility of the receptor's structure. The functional and structural insights obtained on T. pit AstR-C positively assist future efforts in developing environmentally friendly pest control agents that are needed urgently.", 
  "headline": "Structural and Functional Characterization of Allatostatin Receptor Type-C of Thaumetopoea pityocampa, a Potential Target for Next-Generation Pest Control Agents", 
  "identifier": 238024, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Structural and Functional Characterization of Allatostatin Receptor Type-C of Thaumetopoea pityocampa, a Potential Target for Next-Generation Pest Control Agents", 
  "url": "https://aperta.ulakbim.gov.tr/record/238024"
}