1 Ocak 2021 Dergi makalesi Açık Erişim

Kig, Cenk; Mertoglu, Elif; Caliskan, Alper; Agus, Hizlan Hincal; Ucar, Evren Onay; Guler, Varol

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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Kig, Cenk</dc:creator>
  <dc:creator>Mertoglu, Elif</dc:creator>
  <dc:creator>Caliskan, Alper</dc:creator>
  <dc:creator>Agus, Hizlan Hincal</dc:creator>
  <dc:creator>Ucar, Evren Onay</dc:creator>
  <dc:creator>Guler, Varol</dc:creator>
  <dc:date>2021-01-01</dc:date>
  <dc:description>In this study, we compared the effects of terpinolene against the anti-cancer agent cisplatin on proliferation, apoptotic activity and intracellular ROS (reactive oxygen species) production in cancerous (MCF-7) and non-cancerous (HEK-293) cell lines. Cisplatin presented a strong growth-inhibitory effect on both MCF-7 (IC25: 18.77 mu M) and HEK-293 (IC25: 15.03 mu M) cell lines. The growth-inhibitory effect of terpinolene was found to be weaker than cisplatin (IC25: 291.18 mu M for MCF-7 and 345.35 mu M for HEK 293 cells). The expression levels of BAX (by 1.86-fold), cleaved-PARP (by 2.23-fold) and pro-caspase-8 (by 1.74-fold) proteins increased in response to terpinolene treatment in MCF-7 cells. Curiously, the increase in the expression levels of these apoptotic markers was less pronounced in terpinolene-treated non-cancerous HEK 293 cells. Terpinolene, promoted a higher rate of apoptotic and necrotic cell death on MCF-7 cells (apoptotic cells: 25.28 %; necrotic cells: 17.70 %) when compared with HEK-293 cell line (apoptotic cells: 15.47 %; necrotic cells: 9.42 %). Interestingly, terpinolene caused a marked increase (2.05-fold) in intracellular ROS production in MCF-7 cells while HEK-293 cells appeared to be resistant to terpinolene or cisplatin treatments. In conclusion, although terpinolene showed a weaker growth-inhibitory effect on MCF-7 cancer cells, it exhibited a better selectivity than cisplatin in inducing cell death and intracellular oxidative stress in MCF7 breast cancer cells.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/237100</dc:identifier>
  <dc:identifier>oai:aperta.ulakbim.gov.tr:237100</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>BIOLOGIA 76(9) 2757-2766</dc:source>
  <dc:title>Selective and oxidative stress-mediated cell death of MCF-7 cell line induced by terpinolene</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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