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Investigation of the neuroprotective and neuritogenic effects of halotolerant Penicillium flavigenum-derived sorbicillin-like compounds on PC-12 Adh cells

Kaya Tilki, Elif; Engur ozturk, Selin; Ozarda, Mustafa Guclu; Canturk, Zerrin; Dikmen, Miris


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{
  "@context": "https://schema.org/", 
  "@id": 235230, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Anadolu Univ, Fac Pharm, Dept Pharmacol, TR-26210 Eskisehir, Turkey", 
      "name": "Kaya Tilki, Elif"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Pamukkale Univ, Tavas Vocat Sch Hlth Serv, Denizli, Turkey", 
      "name": "Engur ozturk, Selin"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Anadolu Univ, Fac Pharm, Dept Pharmaceut Microbiol, TR-26210 Eskisehir, Turkey", 
      "name": "Ozarda, Mustafa Guclu"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Anadolu Univ, Fac Pharm, Dept Pharmaceut Microbiol, TR-26210 Eskisehir, Turkey", 
      "name": "Canturk, Zerrin"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Anadolu Univ, Fac Pharm, Dept Pharmacol, TR-26210 Eskisehir, Turkey", 
      "name": "Dikmen, Miris"
    }
  ], 
  "datePublished": "2021-01-01", 
  "description": "Parkinson's disease (PD) is an adult-onset neurodegenerative condition caused by oxidative stress and mitochondrial malfunction. In this study, the neuroprotective and neuritogenic activity of water fraction (Sw-fr) containing sorbicillin-like active metabolites of halotolerant P. flavigenum isolated from Salt Lake in Konya, Turkey were investigated on a 6-hydroxydopamine (6-OHDA)-induced PD in vitro PC-12 Adh cell model. Firstly, Sw-fr containing sorbicillin-like active metabolites were extracted from P. flavigenum and was compared with a sorbicillin standard by liquid chromatography-mass spectrometry (LC-MS). Then, the effects of non-cytotoxic concentrations of Sw-fr on the 6-OHDA-induced PD cell model were investigated via real-time cell proliferation analysis using the RTCA DP instrument. The effects of these concentrations on mitochondrial membrane integrity, caspase-3 were investigated by flow cytometry. Neurite outgrowth analysis and immunofluorescence staining were used to explore the neuritogenic effects of neuroprotective doses. By improving PC-12 Adh cell viability, decreasing reactive oxygen species production, and reducing apoptotic cell death, 1 and 10 mu g/mL Sw-fr and sorbicillin standard proved neuroprotective against 6-OHDA-induced neurotoxicity. Furthermore, 1 and 10 mu g/mL Sw-fr significantly induced neurite outgrowth. As a result, sorbicillin-like active metabolites containing Sw-fr were found to have neuroprotective and neuritogenic effects. Sorbicillin-like metabolites obtained from fungi may be novel natural medicines for neurodegenerative diseases.", 
  "headline": "Investigation of the neuroprotective and neuritogenic effects of halotolerant Penicillium flavigenum-derived sorbicillin-like compounds on PC-12 Adh cells", 
  "identifier": 235230, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Investigation of the neuroprotective and neuritogenic effects of halotolerant Penicillium flavigenum-derived sorbicillin-like compounds on PC-12 Adh cells", 
  "url": "https://aperta.ulakbim.gov.tr/record/235230"
}
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