1 Ocak 2021 Dergi makalesi Açık Erişim

Kaya Tilki, Elif; Engur ozturk, Selin; Ozarda, Mustafa Guclu; Canturk, Zerrin; Dikmen, Miris

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/235230</identifier>
  <creators>
    <creator>
      <creatorName>Kaya Tilki, Elif</creatorName>
      <givenName>Elif</givenName>
      <familyName>Kaya Tilki</familyName>
      <affiliation>Anadolu Univ, Fac Pharm, Dept Pharmacol, TR-26210 Eskisehir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Engur ozturk, Selin</creatorName>
      <givenName>Selin</givenName>
      <familyName>Engur ozturk</familyName>
      <affiliation>Pamukkale Univ, Tavas Vocat Sch Hlth Serv, Denizli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ozarda, Mustafa Guclu</creatorName>
      <givenName>Mustafa Guclu</givenName>
      <familyName>Ozarda</familyName>
      <affiliation>Anadolu Univ, Fac Pharm, Dept Pharmaceut Microbiol, TR-26210 Eskisehir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Canturk, Zerrin</creatorName>
      <givenName>Zerrin</givenName>
      <familyName>Canturk</familyName>
      <affiliation>Anadolu Univ, Fac Pharm, Dept Pharmaceut Microbiol, TR-26210 Eskisehir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Dikmen, Miris</creatorName>
      <givenName>Miris</givenName>
      <familyName>Dikmen</familyName>
      <affiliation>Anadolu Univ, Fac Pharm, Dept Pharmacol, TR-26210 Eskisehir, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Investigation Of The Neuroprotective And Neuritogenic Effects Of Halotolerant Penicillium Flavigenum-Derived Sorbicillin-Like Compounds On Pc-12 Adh Cells</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/235230</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1007/s10616-021-00498-9</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Parkinson's disease (PD) is an adult-onset neurodegenerative condition caused by oxidative stress and mitochondrial malfunction. In this study, the neuroprotective and neuritogenic activity of water fraction (Sw-fr) containing sorbicillin-like active metabolites of halotolerant P. flavigenum isolated from Salt Lake in Konya, Turkey were investigated on a 6-hydroxydopamine (6-OHDA)-induced PD in vitro PC-12 Adh cell model. Firstly, Sw-fr containing sorbicillin-like active metabolites were extracted from P. flavigenum and was compared with a sorbicillin standard by liquid chromatography-mass spectrometry (LC-MS). Then, the effects of non-cytotoxic concentrations of Sw-fr on the 6-OHDA-induced PD cell model were investigated via real-time cell proliferation analysis using the RTCA DP instrument. The effects of these concentrations on mitochondrial membrane integrity, caspase-3 were investigated by flow cytometry. Neurite outgrowth analysis and immunofluorescence staining were used to explore the neuritogenic effects of neuroprotective doses. By improving PC-12 Adh cell viability, decreasing reactive oxygen species production, and reducing apoptotic cell death, 1 and 10 mu g/mL Sw-fr and sorbicillin standard proved neuroprotective against 6-OHDA-induced neurotoxicity. Furthermore, 1 and 10 mu g/mL Sw-fr significantly induced neurite outgrowth. As a result, sorbicillin-like active metabolites containing Sw-fr were found to have neuroprotective and neuritogenic effects. Sorbicillin-like metabolites obtained from fungi may be novel natural medicines for neurodegenerative diseases.</description>
  </descriptions>
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