1 Ocak 2021 Dergi makalesi Açık Erişim

Kaya Tilki, Elif; Engur ozturk, Selin; Ozarda, Mustafa Guclu; Canturk, Zerrin; Dikmen, Miris

Citation Style Language JSON

{
  "DOI": "10.1007/s10616-021-00498-9", 
  "abstract": "Parkinson's disease (PD) is an adult-onset neurodegenerative condition caused by oxidative stress and mitochondrial malfunction. In this study, the neuroprotective and neuritogenic activity of water fraction (Sw-fr) containing sorbicillin-like active metabolites of halotolerant P. flavigenum isolated from Salt Lake in Konya, Turkey were investigated on a 6-hydroxydopamine (6-OHDA)-induced PD in vitro PC-12 Adh cell model. Firstly, Sw-fr containing sorbicillin-like active metabolites were extracted from P. flavigenum and was compared with a sorbicillin standard by liquid chromatography-mass spectrometry (LC-MS). Then, the effects of non-cytotoxic concentrations of Sw-fr on the 6-OHDA-induced PD cell model were investigated via real-time cell proliferation analysis using the RTCA DP instrument. The effects of these concentrations on mitochondrial membrane integrity, caspase-3 were investigated by flow cytometry. Neurite outgrowth analysis and immunofluorescence staining were used to explore the neuritogenic effects of neuroprotective doses. By improving PC-12 Adh cell viability, decreasing reactive oxygen species production, and reducing apoptotic cell death, 1 and 10 mu g/mL Sw-fr and sorbicillin standard proved neuroprotective against 6-OHDA-induced neurotoxicity. Furthermore, 1 and 10 mu g/mL Sw-fr significantly induced neurite outgrowth. As a result, sorbicillin-like active metabolites containing Sw-fr were found to have neuroprotective and neuritogenic effects. Sorbicillin-like metabolites obtained from fungi may be novel natural medicines for neurodegenerative diseases.", 
  "author": [
    {
      "family": "Kaya Tilki", 
      "given": " Elif"
    }, 
    {
      "family": "Engur ozturk", 
      "given": " Selin"
    }, 
    {
      "family": "Ozarda", 
      "given": " Mustafa Guclu"
    }, 
    {
      "family": "Canturk", 
      "given": " Zerrin"
    }, 
    {
      "family": "Dikmen", 
      "given": " Miris"
    }
  ], 
  "container_title": "CYTOTECHNOLOGY", 
  "id": "235230", 
  "issue": "6", 
  "issued": {
    "date-parts": [
      [
        2021, 
        1, 
        1
      ]
    ]
  }, 
  "page": "801-813", 
  "title": "Investigation of the neuroprotective and neuritogenic effects of halotolerant Penicillium flavigenum-derived sorbicillin-like compounds on PC-12 Adh cells", 
  "type": "article-journal", 
  "volume": "73"
}