1 Ocak 2021 Dergi makalesi Açık Erişim

Dizaji, Araz Norouz; Ozek, Nihal Simsek; Aysin, Ferhunde; Calis, Ayfer; Yilmaz, Asli; Yilmaz, Mehmet

Dublin Core

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  <dc:creator>Dizaji, Araz Norouz</dc:creator>
  <dc:creator>Ozek, Nihal Simsek</dc:creator>
  <dc:creator>Aysin, Ferhunde</dc:creator>
  <dc:creator>Calis, Ayfer</dc:creator>
  <dc:creator>Yilmaz, Asli</dc:creator>
  <dc:creator>Yilmaz, Mehmet</dc:creator>
  <dc:date>2021-01-01</dc:date>
  <dc:description>This study reports the development of a highly sensitive antibiotic-based discrimination and sensor platform for the detection of Gram-positive bacteria through surface-enhanced Raman spectroscopy (SERS). Herein, a combination of gold nanorod arrays (GNAs) and colloidal gold nanoparticles (AuNPs) was used as a SERS platform. To specifically capture Gram-positive bacteria, both GNAs and AuNPs were functionalized with thiol-modified vancomycin (HS-Van) molecules. Three different strains of bacteria (Bacillus subtilis and Staphylococcus aureus as Gram-positive, and Escherichia coli as Gram-negative) were employed to test the proposed system. HS-Van functionalized GNAs (GNA@Van) captured Gram-positive bacteria with high specificity. Also, the bacteria captured by GNA@Van (GNA@Van@Bct) systems showed high signal-to-noise SERS signals with high reproducibility. Addition of AuNP@Van to GNA@Van@Bct resulted in the emergence of a sandwich system (GNA@Van@Bct@Van@AuNP). This system led to a further enhanced SERS signal. The chemometric analysis of GNA@Van@Bct@Van@AuNP enabled the obvious discrimination and detection of Gram-positive bacteria. For comparison, we also tested a smooth gold surface with the same procedure and a similar trend was observed with lower SERS activity.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/234620</dc:identifier>
  <dc:identifier>oai:aperta.ulakbim.gov.tr:234620</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>ANALYST 146(11) 3642-3653</dc:source>
  <dc:title>Combining vancomycin-modified gold nanorod arrays and colloidal nanoparticles as a sandwich model for the discrimination of Gram-positive bacteria and their detection via surface-enhanced Raman spectroscopy (SERS)</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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