1 Ocak 2021 Dergi makalesi Açık Erişim

Dizaji, Araz Norouz; Ozek, Nihal Simsek; Aysin, Ferhunde; Calis, Ayfer; Yilmaz, Asli; Yilmaz, Mehmet

DataCite XML

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/234620</identifier>
  <creators>
    <creator>
      <creatorName>Dizaji, Araz Norouz</creatorName>
      <givenName>Araz Norouz</givenName>
      <familyName>Dizaji</familyName>
    </creator>
    <creator>
      <creatorName>Ozek, Nihal Simsek</creatorName>
      <givenName>Nihal Simsek</givenName>
      <familyName>Ozek</familyName>
    </creator>
    <creator>
      <creatorName>Aysin, Ferhunde</creatorName>
      <givenName>Ferhunde</givenName>
      <familyName>Aysin</familyName>
    </creator>
    <creator>
      <creatorName>Calis, Ayfer</creatorName>
      <givenName>Ayfer</givenName>
      <familyName>Calis</familyName>
      <affiliation>Giresun Univ, Dept Genet &amp; Bioengn, TR-28200 Giresun, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Yilmaz, Asli</creatorName>
      <givenName>Asli</givenName>
      <familyName>Yilmaz</familyName>
    </creator>
    <creator>
      <creatorName>Yilmaz, Mehmet</creatorName>
      <givenName>Mehmet</givenName>
      <familyName>Yilmaz</familyName>
    </creator>
  </creators>
  <titles>
    <title>Combining Vancomycin-Modified Gold Nanorod Arrays And Colloidal Nanoparticles As A Sandwich Model For The Discrimination Of Gram-Positive Bacteria And Their Detection Via Surface-Enhanced Raman Spectroscopy (Sers)</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/234620</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1039/d1an00321f</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">This study reports the development of a highly sensitive antibiotic-based discrimination and sensor platform for the detection of Gram-positive bacteria through surface-enhanced Raman spectroscopy (SERS). Herein, a combination of gold nanorod arrays (GNAs) and colloidal gold nanoparticles (AuNPs) was used as a SERS platform. To specifically capture Gram-positive bacteria, both GNAs and AuNPs were functionalized with thiol-modified vancomycin (HS-Van) molecules. Three different strains of bacteria (Bacillus subtilis and Staphylococcus aureus as Gram-positive, and Escherichia coli as Gram-negative) were employed to test the proposed system. HS-Van functionalized GNAs (GNA@Van) captured Gram-positive bacteria with high specificity. Also, the bacteria captured by GNA@Van (GNA@Van@Bct) systems showed high signal-to-noise SERS signals with high reproducibility. Addition of AuNP@Van to GNA@Van@Bct resulted in the emergence of a sandwich system (GNA@Van@Bct@Van@AuNP). This system led to a further enhanced SERS signal. The chemometric analysis of GNA@Van@Bct@Van@AuNP enabled the obvious discrimination and detection of Gram-positive bacteria. For comparison, we also tested a smooth gold surface with the same procedure and a similar trend was observed with lower SERS activity.</description>
  </descriptions>
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