1 Ocak 2021 Dergi makalesi Açık Erişim

Dizaji, Araz Norouz; Ozek, Nihal Simsek; Aysin, Ferhunde; Calis, Ayfer; Yilmaz, Asli; Yilmaz, Mehmet

Citation Style Language JSON

{
  "DOI": "10.1039/d1an00321f", 
  "abstract": "This study reports the development of a highly sensitive antibiotic-based discrimination and sensor platform for the detection of Gram-positive bacteria through surface-enhanced Raman spectroscopy (SERS). Herein, a combination of gold nanorod arrays (GNAs) and colloidal gold nanoparticles (AuNPs) was used as a SERS platform. To specifically capture Gram-positive bacteria, both GNAs and AuNPs were functionalized with thiol-modified vancomycin (HS-Van) molecules. Three different strains of bacteria (Bacillus subtilis and Staphylococcus aureus as Gram-positive, and Escherichia coli as Gram-negative) were employed to test the proposed system. HS-Van functionalized GNAs (GNA@Van) captured Gram-positive bacteria with high specificity. Also, the bacteria captured by GNA@Van (GNA@Van@Bct) systems showed high signal-to-noise SERS signals with high reproducibility. Addition of AuNP@Van to GNA@Van@Bct resulted in the emergence of a sandwich system (GNA@Van@Bct@Van@AuNP). This system led to a further enhanced SERS signal. The chemometric analysis of GNA@Van@Bct@Van@AuNP enabled the obvious discrimination and detection of Gram-positive bacteria. For comparison, we also tested a smooth gold surface with the same procedure and a similar trend was observed with lower SERS activity.", 
  "author": [
    {
      "family": "Dizaji", 
      "given": " Araz Norouz"
    }, 
    {
      "family": "Ozek", 
      "given": " Nihal Simsek"
    }, 
    {
      "family": "Aysin", 
      "given": " Ferhunde"
    }, 
    {
      "family": "Calis", 
      "given": " Ayfer"
    }, 
    {
      "family": "Yilmaz", 
      "given": " Asli"
    }, 
    {
      "family": "Yilmaz", 
      "given": " Mehmet"
    }
  ], 
  "container_title": "ANALYST", 
  "id": "234620", 
  "issue": "11", 
  "issued": {
    "date-parts": [
      [
        2021, 
        1, 
        1
      ]
    ]
  }, 
  "page": "3642-3653", 
  "title": "Combining vancomycin-modified gold nanorod arrays and colloidal nanoparticles as a sandwich model for the discrimination of Gram-positive bacteria and their detection via surface-enhanced Raman spectroscopy (SERS)", 
  "type": "article-journal", 
  "volume": "146"
}