1 Ocak 2021 Dergi makalesi Açık Erişim

Yakubogullari, Nilgun; Coven, Furkan Ozan; Cebi, Nusin; Coven, Fethiye; Coven, Nejdet; Genc, Rukan; Bedir, Erdal; Nalbantsoy, Ayse

Citation Style Language JSON

{
  "DOI": "10.1016/j.biologicals.2021.01.005", 
  "abstract": "Astragaloside VII (AST-VII), a major cycloartane saponin isolated from Turkish Astragalus species, turned out to be one of the most active metabolites demonstrating Th1/Th2 balanced immune response. As Quillaja saponins are extensively used in adjuvant systems, this study made an attempt to improve AST-VII based adjuvant systems by using different immunostimulatory/delivery agents (monophosphoryllipid A (MPL), Astragalus polysaccharide (APS) and squalene) and to induce cellular and humoral immune response against a viral vaccine. For this purpose, Newcastle Disease vaccine (NDV) was chosen as a model vaccine. Swiss albino mice were immunized subcutaneously with LaSota vaccines in the presence/absence of AST-VII or developed adjuvant systems. AST-VII administration both in live/inactivated LaSota vaccines induced neutralizing and NDV specific IgG, IgG1 and IgG2b antibodies response as well as IL-2 and IL-4 production. APS based delivery systems enhanced the production of neutralizing antibody and the minor augmentation of IFN-? and IL-2 levels. Squalene emulsion (SE) alone or combined with AST-VII were effective in NDV restimulated splenocyte proliferation. As a conclusion, AST-VII and AST-VII containing adjuvant systems demonstrated Th1/Th2 balanced antibody and cellular immune responses in NDV vaccines. Thus, these systems could be developed as vaccine adjuvants in viral vaccines as alternative to saponin-based adjuvants.", 
  "author": [
    {
      "family": "Yakubogullari", 
      "given": " Nilgun"
    }, 
    {
      "family": "Coven", 
      "given": " Furkan Ozan"
    }, 
    {
      "family": "Cebi", 
      "given": " Nusin"
    }, 
    {
      "family": "Coven", 
      "given": " Fethiye"
    }, 
    {
      "family": "Coven", 
      "given": " Nejdet"
    }, 
    {
      "family": "Genc", 
      "given": " Rukan"
    }, 
    {
      "family": "Bedir", 
      "given": " Erdal"
    }, 
    {
      "family": "Nalbantsoy", 
      "given": " Ayse"
    }
  ], 
  "container_title": "BIOLOGICALS", 
  "id": "233224", 
  "issued": {
    "date-parts": [
      [
        2021, 
        1, 
        1
      ]
    ]
  }, 
  "page": "28-37", 
  "title": "Evaluation of adjuvant activity of Astragaloside VII and its combination with different immunostimulating agents in Newcastle Disease vaccine", 
  "type": "article-journal", 
  "volume": "70"
}