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Boronic Acid Substituted Polyaniline Based Enzymatic Biosensor System for Catechol Detection

   Kalkan, Zeycan; Yence, Merve; Turk, Fatih; Bektas, Tamer U.; Ozturk, Soner; Surdem, Sedat; Yildirim-Tirgil, Nimet

In this research, an electrochemical enzymatic biosensor system for sensitive and selective catechol detection was developed with using boric acid substituted Polyaniline film coated two different electrodes; gold screen-printed electrode and glassy carbon electrode. Electropolymerization of 3-aminophenylboronic acid is carried out through various electropolymerization conditions, and the best conditions for biosensors systems are concluded. The developed tyrosinase enzyme-based biosensor system is used for the determination of catechol. The limit of detections(LOD) of the developed biosensor was 0.25 mu M and 1.8 mu M with Au SPE and GCE systems, respectively. Additionally, real sample analysis was also performed for controlled catechol added green tea samples.

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