1 Ocak 2013 Dergi makalesi Açık Erişim

Kelestimur, Haluk; Kacar, Emine; Uzun, Aysegul; Ozcan, Mete; Kutlu, Selim

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{
  "@context": "https://schema.org/", 
  "@id": 12881, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Firat Univ, Sch Med, Dept Physiol, TR-23119 Elazig, Turkey", 
      "name": "Kelestimur, Haluk"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Firat Univ, Sch Med, Dept Physiol, TR-23119 Elazig, Turkey", 
      "name": "Kacar, Emine"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Karadeniz Tech Univ, Sch Med, Dept Biochem, Elazig, Turkey", 
      "name": "Uzun, Aysegul"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Firat Univ, Sch Med, Dept Biophys, TR-23119 Elazig, Turkey", 
      "name": "Ozcan, Mete"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Firat Univ, Sch Med, Dept Physiol, TR-23119 Elazig, Turkey", 
      "name": "Kutlu, Selim"
    }
  ], 
  "datePublished": "2013-01-01", 
  "description": "The hypothalamic Arg-Phe-amide-related peptides, gonadotropin-inhibitory hormone and orthologous mammalian peptides of Arg-Phe-amide, may be important regulators of the hypothalamus-pituitary-gonadal reproductive axis. These peptides may modulate the effects of kisspeptins because they are presently recognized as the most potent activators of the hypothalamus-pituitary-gonadal axis. However, their effects on gonadotropin-releasing hormone neurons have not been investigated. In the current study, the GT1-7 cell line-expressing gonadotropin-releasing hormone was used as a model to explore the effects of Arg-Phe-amide-related peptides on kisspeptin activation. Intracellular calcium concentration was quantified using the calcium-sensitive dye, fura-2 acetoxymethyl ester. Gonadotropin-releasing hormone released into the medium was detected via enzyme-linked immunosorbent assay. Results showed that 100 nmol/L kisspeptin-10 significantly increased gonadotropin-releasing hormone levels (at 120 minutes of exposure) and intracellular calcium concentrations. Co-treatment of kisspeptin with 1 mu mol/L gonadotropin-inhibitory hormone or 1 mu mol/L Arg-Phe-amide-related peptide-1 significantly attenuated levels of kisspeptin-induced gonadotropin-releasing hormone but did not affect kisspeptin-induced elevations of intracellular calcium concentration. Overall, the results suggest that gonadotropin-inhibitory hormone and Arg-Phe-amide-related peptide-1 may have inhibitory effects on kisspeptin-activated gonadotropin-releasing hormone neurons independent of the calcium signaling pathway.", 
  "headline": "Arg-Phe-amide-related peptides influence gonadotropin-releasing hormone neurons(star.)", 
  "identifier": 12881, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Arg-Phe-amide-related peptides influence gonadotropin-releasing hormone neurons(star.)", 
  "url": "https://aperta.ulakbim.gov.tr/record/12881"
}