1 Ocak 2013 Dergi makalesi Açık Erişim

Kelestimur, Haluk; Kacar, Emine; Uzun, Aysegul; Ozcan, Mete; Kutlu, Selim

Citation Style Language JSON

{
  "DOI": "10.3969/j.issn.1673-5374.2013.18.009", 
  "abstract": "The hypothalamic Arg-Phe-amide-related peptides, gonadotropin-inhibitory hormone and orthologous mammalian peptides of Arg-Phe-amide, may be important regulators of the hypothalamus-pituitary-gonadal reproductive axis. These peptides may modulate the effects of kisspeptins because they are presently recognized as the most potent activators of the hypothalamus-pituitary-gonadal axis. However, their effects on gonadotropin-releasing hormone neurons have not been investigated. In the current study, the GT1-7 cell line-expressing gonadotropin-releasing hormone was used as a model to explore the effects of Arg-Phe-amide-related peptides on kisspeptin activation. Intracellular calcium concentration was quantified using the calcium-sensitive dye, fura-2 acetoxymethyl ester. Gonadotropin-releasing hormone released into the medium was detected via enzyme-linked immunosorbent assay. Results showed that 100 nmol/L kisspeptin-10 significantly increased gonadotropin-releasing hormone levels (at 120 minutes of exposure) and intracellular calcium concentrations. Co-treatment of kisspeptin with 1 mu mol/L gonadotropin-inhibitory hormone or 1 mu mol/L Arg-Phe-amide-related peptide-1 significantly attenuated levels of kisspeptin-induced gonadotropin-releasing hormone but did not affect kisspeptin-induced elevations of intracellular calcium concentration. Overall, the results suggest that gonadotropin-inhibitory hormone and Arg-Phe-amide-related peptide-1 may have inhibitory effects on kisspeptin-activated gonadotropin-releasing hormone neurons independent of the calcium signaling pathway.", 
  "author": [
    {
      "family": "Kelestimur", 
      "given": " Haluk"
    }, 
    {
      "family": "Kacar", 
      "given": " Emine"
    }, 
    {
      "family": "Uzun", 
      "given": " Aysegul"
    }, 
    {
      "family": "Ozcan", 
      "given": " Mete"
    }, 
    {
      "family": "Kutlu", 
      "given": " Selim"
    }
  ], 
  "container_title": "NEURAL REGENERATION RESEARCH", 
  "id": "12881", 
  "issue": "18", 
  "issued": {
    "date-parts": [
      [
        2013, 
        1, 
        1
      ]
    ]
  }, 
  "page": "1714-1720", 
  "title": "Arg-Phe-amide-related peptides influence gonadotropin-releasing hormone neurons(star.)", 
  "type": "article-journal", 
  "volume": "8"
}