1 Ocak 2013 Dergi makalesi Açık Erişim

Kelestimur, Haluk; Kacar, Emine; Uzun, Aysegul; Ozcan, Mete; Kutlu, Selim

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    "creators": [
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        "affiliation": "Firat Univ, Sch Med, Dept Physiol, TR-23119 Elazig, Turkey", 
        "name": "Kelestimur, Haluk"
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      {
        "affiliation": "Firat Univ, Sch Med, Dept Physiol, TR-23119 Elazig, Turkey", 
        "name": "Kacar, Emine"
      }, 
      {
        "affiliation": "Karadeniz Tech Univ, Sch Med, Dept Biochem, Elazig, Turkey", 
        "name": "Uzun, Aysegul"
      }, 
      {
        "affiliation": "Firat Univ, Sch Med, Dept Biophys, TR-23119 Elazig, Turkey", 
        "name": "Ozcan, Mete"
      }, 
      {
        "affiliation": "Firat Univ, Sch Med, Dept Physiol, TR-23119 Elazig, Turkey", 
        "name": "Kutlu, Selim"
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    "description": "The hypothalamic Arg-Phe-amide-related peptides, gonadotropin-inhibitory hormone and orthologous mammalian peptides of Arg-Phe-amide, may be important regulators of the hypothalamus-pituitary-gonadal reproductive axis. These peptides may modulate the effects of kisspeptins because they are presently recognized as the most potent activators of the hypothalamus-pituitary-gonadal axis. However, their effects on gonadotropin-releasing hormone neurons have not been investigated. In the current study, the GT1-7 cell line-expressing gonadotropin-releasing hormone was used as a model to explore the effects of Arg-Phe-amide-related peptides on kisspeptin activation. Intracellular calcium concentration was quantified using the calcium-sensitive dye, fura-2 acetoxymethyl ester. Gonadotropin-releasing hormone released into the medium was detected via enzyme-linked immunosorbent assay. Results showed that 100 nmol/L kisspeptin-10 significantly increased gonadotropin-releasing hormone levels (at 120 minutes of exposure) and intracellular calcium concentrations. Co-treatment of kisspeptin with 1 mu mol/L gonadotropin-inhibitory hormone or 1 mu mol/L Arg-Phe-amide-related peptide-1 significantly attenuated levels of kisspeptin-induced gonadotropin-releasing hormone but did not affect kisspeptin-induced elevations of intracellular calcium concentration. Overall, the results suggest that gonadotropin-inhibitory hormone and Arg-Phe-amide-related peptide-1 may have inhibitory effects on kisspeptin-activated gonadotropin-releasing hormone neurons independent of the calcium signaling pathway.", 
    "doi": "10.3969/j.issn.1673-5374.2013.18.009", 
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    "journal": {
      "issue": "18", 
      "pages": "1714-1720", 
      "title": "NEURAL REGENERATION RESEARCH", 
      "volume": "8"
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      "title": "Dergi makalesi", 
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    "title": "Arg-Phe-amide-related peptides influence gonadotropin-releasing hormone neurons(star.)"
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