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Assessment of poly(3-hydroxybutyrate) synthesis from a novel obligate alkaliphilic Bacillus marmarensis and generation of its composite scaffold via electrospinning

Ozgoren, Tugba; Pinar, Orkun; Bozdag, Gulnihal; Denizci, Aziz Akin; Gunduz, Oguzhan; Hatir, Pinar Cakir; Kazan, Dilek


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        "name": "Ozgoren, Tugba"
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      {
        "affiliation": "Marmara Univ, Fac Engn, Dept Bioengn, Goztepe Campus, TR-34722 Istanbul, Turkey", 
        "name": "Pinar, Orkun"
      }, 
      {
        "affiliation": "Marmara Univ, Fac Engn, Dept Bioengn, Goztepe Campus, TR-34722 Istanbul, Turkey", 
        "name": "Bozdag, Gulnihal"
      }, 
      {
        "affiliation": "Sci & Technol Res Council Turkey, Marmara Res Ctr, Genet Engn & Biotechnol Inst, POB 21, TR-41470 Gebze, Kocaeli, Turkey", 
        "name": "Denizci, Aziz Akin"
      }, 
      {
        "affiliation": "Marmara Univ, Fac Technol, Dept Met & Mat Engn, Goztepe Campus, TR-34722 Istanbul, Turkey", 
        "name": "Gunduz, Oguzhan"
      }, 
      {
        "affiliation": "Istanbul Arel Univ, Dept Biomed Engn, Fac Engn & Architecture, TR-34537 Istanbul, Turkey", 
        "name": "Hatir, Pinar Cakir"
      }, 
      {
        "affiliation": "Marmara Univ, Fac Engn, Dept Bioengn, Goztepe Campus, TR-34722 Istanbul, Turkey", 
        "name": "Kazan, Dilek"
      }
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    "description": "In this study, poly(3-hydroxybutyrate) (PHB) production from a newly isolated obligate alkaliphilic Bacillus marmarensis DSM 21297 was investigated to evaluate the ability of obligate alkaliphilic strain to produce a biopolymer. Additionally, electrospun nanofibers from B. marmarensis PHB (Bm-PHB) were generated using Bm-PHB/polycaprolactone (PCL) blend to evaluate the applicability of Bm-PHB. According to the experimental results, the metabolic activity of B. marmarensis decreased the pH of the medium by generating H+ ions to initiate Bm-PHB production, which was achieved at pH below 9.0. Regarding medium components, the addition of MgSO4 center dot 7H(2)O and KH2PO4 to the medium containing 1% glucose enhanced the amount of Bm-PHB synthesis, and an approximately 60% increase in PHB concentration was obtained in the presence of mineral salts.", 
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      "pages": "982-991", 
      "title": "INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES", 
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    "title": "Assessment of poly(3-hydroxybutyrate) synthesis from a novel obligate alkaliphilic Bacillus marmarensis and generation of its composite scaffold via electrospinning"
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