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Assessment of poly(3-hydroxybutyrate) synthesis from a novel obligate alkaliphilic Bacillus marmarensis and generation of its composite scaffold via electrospinning

Ozgoren, Tugba; Pinar, Orkun; Bozdag, Gulnihal; Denizci, Aziz Akin; Gunduz, Oguzhan; Hatir, Pinar Cakir; Kazan, Dilek


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{
  "DOI": "10.1016/j.ijbiomac.2018.08.014", 
  "abstract": "In this study, poly(3-hydroxybutyrate) (PHB) production from a newly isolated obligate alkaliphilic Bacillus marmarensis DSM 21297 was investigated to evaluate the ability of obligate alkaliphilic strain to produce a biopolymer. Additionally, electrospun nanofibers from B. marmarensis PHB (Bm-PHB) were generated using Bm-PHB/polycaprolactone (PCL) blend to evaluate the applicability of Bm-PHB. According to the experimental results, the metabolic activity of B. marmarensis decreased the pH of the medium by generating H+ ions to initiate Bm-PHB production, which was achieved at pH below 9.0. Regarding medium components, the addition of MgSO4 center dot 7H(2)O and KH2PO4 to the medium containing 1% glucose enhanced the amount of Bm-PHB synthesis, and an approximately 60% increase in PHB concentration was obtained in the presence of mineral salts.", 
  "author": [
    {
      "family": "Ozgoren", 
      "given": " Tugba"
    }, 
    {
      "family": "Pinar", 
      "given": " Orkun"
    }, 
    {
      "family": "Bozdag", 
      "given": " Gulnihal"
    }, 
    {
      "family": "Denizci", 
      "given": " Aziz Akin"
    }, 
    {
      "family": "Gunduz", 
      "given": " Oguzhan"
    }, 
    {
      "family": "Hatir", 
      "given": " Pinar Cakir"
    }, 
    {
      "family": "Kazan", 
      "given": " Dilek"
    }
  ], 
  "container_title": "INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES", 
  "id": "113166", 
  "issued": {
    "date-parts": [
      [
        2018, 
        1, 
        1
      ]
    ]
  }, 
  "page": "982-991", 
  "title": "Assessment of poly(3-hydroxybutyrate) synthesis from a novel obligate alkaliphilic Bacillus marmarensis and generation of its composite scaffold via electrospinning", 
  "type": "article-journal", 
  "volume": "119"
}
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