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Assessment of poly(3-hydroxybutyrate) synthesis from a novel obligate alkaliphilic Bacillus marmarensis and generation of its composite scaffold via electrospinning

Ozgoren, Tugba; Pinar, Orkun; Bozdag, Gulnihal; Denizci, Aziz Akin; Gunduz, Oguzhan; Hatir, Pinar Cakir; Kazan, Dilek


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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/113166</identifier>
  <creators>
    <creator>
      <creatorName>Ozgoren, Tugba</creatorName>
      <givenName>Tugba</givenName>
      <familyName>Ozgoren</familyName>
    </creator>
    <creator>
      <creatorName>Pinar, Orkun</creatorName>
      <givenName>Orkun</givenName>
      <familyName>Pinar</familyName>
      <affiliation>Marmara Univ, Fac Engn, Dept Bioengn, Goztepe Campus, TR-34722 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Bozdag, Gulnihal</creatorName>
      <givenName>Gulnihal</givenName>
      <familyName>Bozdag</familyName>
      <affiliation>Marmara Univ, Fac Engn, Dept Bioengn, Goztepe Campus, TR-34722 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Denizci, Aziz Akin</creatorName>
      <givenName>Aziz Akin</givenName>
      <familyName>Denizci</familyName>
      <affiliation>Sci &amp; Technol Res Council Turkey, Marmara Res Ctr, Genet Engn &amp; Biotechnol Inst, POB 21, TR-41470 Gebze, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Gunduz, Oguzhan</creatorName>
      <givenName>Oguzhan</givenName>
      <familyName>Gunduz</familyName>
      <affiliation>Marmara Univ, Fac Technol, Dept Met &amp; Mat Engn, Goztepe Campus, TR-34722 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Hatir, Pinar Cakir</creatorName>
      <givenName>Pinar Cakir</givenName>
      <familyName>Hatir</familyName>
      <affiliation>Istanbul Arel Univ, Dept Biomed Engn, Fac Engn &amp; Architecture, TR-34537 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Kazan, Dilek</creatorName>
      <givenName>Dilek</givenName>
      <familyName>Kazan</familyName>
      <affiliation>Marmara Univ, Fac Engn, Dept Bioengn, Goztepe Campus, TR-34722 Istanbul, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Assessment Of Poly(3-Hydroxybutyrate) Synthesis From A Novel Obligate Alkaliphilic Bacillus Marmarensis And Generation Of Its Composite Scaffold Via Electrospinning</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2018</publicationYear>
  <dates>
    <date dateType="Issued">2018-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/113166</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.ijbiomac.2018.08.014</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">In this study, poly(3-hydroxybutyrate) (PHB) production from a newly isolated obligate alkaliphilic Bacillus marmarensis DSM 21297 was investigated to evaluate the ability of obligate alkaliphilic strain to produce a biopolymer. Additionally, electrospun nanofibers from B. marmarensis PHB (Bm-PHB) were generated using Bm-PHB/polycaprolactone (PCL) blend to evaluate the applicability of Bm-PHB. According to the experimental results, the metabolic activity of B. marmarensis decreased the pH of the medium by generating H+ ions to initiate Bm-PHB production, which was achieved at pH below 9.0. Regarding medium components, the addition of MgSO4 center dot 7H(2)O and KH2PO4 to the medium containing 1% glucose enhanced the amount of Bm-PHB synthesis, and an approximately 60% increase in PHB concentration was obtained in the presence of mineral salts.</description>
  </descriptions>
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