1 Ocak 2014 Dergi makalesi Açık Erişim

Tang, Zhi; Baykal, Ahmet Tank; Gao, Hui; Quezada, Hernan Concha; Zhang, Haiyan; Bereczki, Erika; Serhatli, Muge; Baykal, Betul; Acioglu, Cigdem; Wang, Shan; Ioja, Eniko; Ji, Xinying; Zhang, Yan; Guan, Zhizhong; Winblad, Bengt; Pei, Jin-Jing

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    "creators": [
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        "affiliation": "Karolinska Inst, KI Alzheimer Dis Res Ctr, SE-14186 Huddinge, Sweden", 
        "name": "Tang, Zhi"
      }, 
      {
        "affiliation": "Istanbul Medipol Univ, Sch Med, Dept Med Biochem, TR-34083 Unkapani, Fatih Istanbul, Turkey", 
        "name": "Baykal, Ahmet Tank"
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      {
        "affiliation": "Karolinska Inst, Dept Biosci & Nutr, SE-14157 Huddinge, Sweden", 
        "name": "Gao, Hui"
      }, 
      {
        "affiliation": "Karolinska Inst Hosp, Ctr Infect Med, Dept Med, Huddinge, Sweden", 
        "name": "Quezada, Hernan Concha"
      }, 
      {
        "affiliation": "Karolinska Inst, KI Alzheimer Dis Res Ctr, SE-14186 Huddinge, Sweden", 
        "name": "Zhang, Haiyan"
      }, 
      {
        "affiliation": "Karolinska Inst, KI Alzheimer Dis Res Ctr, SE-14186 Huddinge, Sweden", 
        "name": "Bereczki, Erika"
      }, 
      {
        "affiliation": "TUBITAK, Marmara Res Ctr, Genet Engn & Biotechnol Inst, TR-41470 Gebze, Turkey", 
        "name": "Serhatli, Muge"
      }, 
      {
        "affiliation": "TUBITAK, Marmara Res Ctr, Genet Engn & Biotechnol Inst, TR-41470 Gebze, Turkey", 
        "name": "Baykal, Betul"
      }, 
      {
        "affiliation": "TUBITAK, Marmara Res Ctr, Genet Engn & Biotechnol Inst, TR-41470 Gebze, Turkey", 
        "name": "Acioglu, Cigdem"
      }, 
      {
        "affiliation": "Karolinska Inst, KI Alzheimer Dis Res Ctr, SE-14186 Huddinge, Sweden", 
        "name": "Wang, Shan"
      }, 
      {
        "affiliation": "Karolinska Inst, KI Alzheimer Dis Res Ctr, SE-14186 Huddinge, Sweden", 
        "name": "Ioja, Eniko"
      }, 
      {
        "affiliation": "Henan Univ, Sch Med, Inst Immunol, Henan Prov Key Med Lab Cellular & Mol Immunol, Kaifeng 475004, Henan, Peoples R China", 
        "name": "Ji, Xinying"
      }, 
      {
        "affiliation": "Karolinska Inst, KI Alzheimer Dis Res Ctr, SE-14186 Huddinge, Sweden", 
        "name": "Zhang, Yan"
      }, 
      {
        "name": "Guan, Zhizhong"
      }, 
      {
        "affiliation": "Karolinska Inst, KI Alzheimer Dis Res Ctr, SE-14186 Huddinge, Sweden", 
        "name": "Winblad, Bengt"
      }, 
      {
        "affiliation": "Karolinska Inst, KI Alzheimer Dis Res Ctr, SE-14186 Huddinge, Sweden", 
        "name": "Pei, Jin-Jing"
      }
    ], 
    "description": "mTor plays a central role in controlling protein homeostasis and cell survival. Recently, we have demonstrated that perturbations of mTor signaling are implicated in Alzheimer's disease (AD) and that mTor complex 1 (mTorC1) is involved in the formation of toxic phospho-tau. Therefore, we employed mass-spectrometry-based proteomics to identify specific protein expression changes in relation with cell survival in human neuroblastoma SH-SYSY cells expressing genetically modified mTor. Cell death in SH-SYSY cells was induced by moderate serum deprivation. Using flow cytometry we observed that up-regulated mTor complex 2 (mTorC2) increases the number of viable cells. By using a combination approach of proteomic and enrichment analysis we have identified several proteins (Thioredoxin-dependent peroxide reductase, Peroxiredoxin-5, Cofilin 1 (non-muscle), Annexin A5, Mortalin, and 14-3-3 protein zeta/delta) involved in mitochondrial integrity, apoptotosis, and pro-survival functions (caspase inhibitor activity and anti-apoptosis) that were significantly altered by mTor activity modulation. The major findings of this study are the implication of mTorC2 but not mTorC1 in cell viability modulation by activating the pro-survival machinery. Taken together, these results suggest that up-regulated mTorC2 might be playing an important role in promoting cell survival by suppressing the mitochondria-caspase-apoptotic pathway in vitro.", 
    "doi": "10.1021/pr500192g", 
    "has_grant": false, 
    "journal": {
      "issue": "5", 
      "pages": "2433-2444", 
      "title": "JOURNAL OF PROTEOME RESEARCH", 
      "volume": "13"
    }, 
    "license": {
      "id": "cc-by"
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    "publication_date": "2014-01-01", 
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      "title": "Dergi makalesi", 
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    "title": "mTor Is a Signaling Hub in Cell Survival: A Mass-Spectrometry-Based Proteomics Investigation"
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