Dergi makalesi Açık Erişim
Tang, Zhi; Baykal, Ahmet Tank; Gao, Hui; Quezada, Hernan Concha; Zhang, Haiyan; Bereczki, Erika; Serhatli, Muge; Baykal, Betul; Acioglu, Cigdem; Wang, Shan; Ioja, Eniko; Ji, Xinying; Zhang, Yan; Guan, Zhizhong; Winblad, Bengt; Pei, Jin-Jing
{ "DOI": "10.1021/pr500192g", "abstract": "mTor plays a central role in controlling protein homeostasis and cell survival. Recently, we have demonstrated that perturbations of mTor signaling are implicated in Alzheimer's disease (AD) and that mTor complex 1 (mTorC1) is involved in the formation of toxic phospho-tau. Therefore, we employed mass-spectrometry-based proteomics to identify specific protein expression changes in relation with cell survival in human neuroblastoma SH-SYSY cells expressing genetically modified mTor. Cell death in SH-SYSY cells was induced by moderate serum deprivation. Using flow cytometry we observed that up-regulated mTor complex 2 (mTorC2) increases the number of viable cells. By using a combination approach of proteomic and enrichment analysis we have identified several proteins (Thioredoxin-dependent peroxide reductase, Peroxiredoxin-5, Cofilin 1 (non-muscle), Annexin A5, Mortalin, and 14-3-3 protein zeta/delta) involved in mitochondrial integrity, apoptotosis, and pro-survival functions (caspase inhibitor activity and anti-apoptosis) that were significantly altered by mTor activity modulation. The major findings of this study are the implication of mTorC2 but not mTorC1 in cell viability modulation by activating the pro-survival machinery. Taken together, these results suggest that up-regulated mTorC2 might be playing an important role in promoting cell survival by suppressing the mitochondria-caspase-apoptotic pathway in vitro.", "author": [ { "family": "Tang", "given": " Zhi" }, { "family": "Baykal", "given": " Ahmet Tank" }, { "family": "Gao", "given": " Hui" }, { "family": "Quezada", "given": " Hernan Concha" }, { "family": "Zhang", "given": " Haiyan" }, { "family": "Bereczki", "given": " Erika" }, { "family": "Serhatli", "given": " Muge" }, { "family": "Baykal", "given": " Betul" }, { "family": "Acioglu", "given": " Cigdem" }, { "family": "Wang", "given": " Shan" }, { "family": "Ioja", "given": " Eniko" }, { "family": "Ji", "given": " Xinying" }, { "family": "Zhang", "given": " Yan" }, { "family": "Guan", "given": " Zhizhong" }, { "family": "Winblad", "given": " Bengt" }, { "family": "Pei", "given": " Jin-Jing" } ], "container_title": "JOURNAL OF PROTEOME RESEARCH", "id": "99259", "issue": "5", "issued": { "date-parts": [ [ 2014, 1, 1 ] ] }, "page": "2433-2444", "title": "mTor Is a Signaling Hub in Cell Survival: A Mass-Spectrometry-Based Proteomics Investigation", "type": "article-journal", "volume": "13" }
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