1 Ocak 2003 Dergi makalesi Açık Erişim

Arat, S; Rzucidlo, SJ; Stice, SL

Citation Style Language JSON

{
  "DOI": "10.1002/mrd.10362", 
  "abstract": "This study examined the chromatin morphology, in vitro development, and expression of selected genes in cloned embryos produced by transfer of mouse embryonic fibroblasts (MEF) into the bovine ooplasm. After 6 hr of activation, inter-species nuclear transfer (NT) embryos (MEF-NT) had one (70%) or two pronuclei (20%), respectively. After 72 hr of culture in vitro, 62.6% of the MEF-NTs were arrested at the 8-cell stage, 31.2% reached the 2- to 4-cell stage, and only 6.2% had more than eight blastomeres, but none of these developed to the blastocyst stage. Whereas, 20% of NT embryos derived from bovine embryonic fibroblast fused with bovine ooplasm (BEF-NT) reached the blastocyst stage. Donor MEF nuclei expressing an Enhanced Green Fluorescent Protein (EGFP) transgene resulted in 1- to 8-cell stage MEF-NT that expressed EGFP. The expression of selected genes was examined in 8-cell MEF-NTs, 8-cell mouse embryos, enucleated bovine oocytes, and MEFs using RT-PCR. The mRNA for heat shock protein 70.1 (Hsp 70.1) gene was detected in MEF-NTs and MEF, but not in mouse embryos. The hydroxy-phosphoribosyl transferase (HPRT) mRNA was found in normal mouse embryos and MEF but not in MEF-NTs. Expression of Oct-4 and embryonic alkaline phospatase (eAP) genes was only detected in normal mouse embryos and not in the inter-species NT embryos. Abnormal gene-expression profiles were associated with an arrest in the development at the 8-cell stage, but MEF-NT embryos appeared to have progressed through gross chromatin remodeling, typical of intra-species NT embryos. Therefore, molecular reprogramming rather than chromatin remodeling may be a better indicator of nuclear reprogramming in inter-species NT embryos. (C) 2003 Wiley-Liss, Inc.", 
  "author": [
    {
      "family": "Arat", 
      "given": " S"
    }, 
    {
      "family": "Rzucidlo", 
      "given": " SJ"
    }, 
    {
      "family": "Stice", 
      "given": " SL"
    }
  ], 
  "container_title": "MOLECULAR REPRODUCTION AND DEVELOPMENT", 
  "id": "97747", 
  "issue": "4", 
  "issued": {
    "date-parts": [
      [
        2003, 
        1, 
        1
      ]
    ]
  }, 
  "page": "334-342", 
  "title": "Gene expression and in vitro development of inter-species nuclear transfer embryos", 
  "type": "article-journal", 
  "volume": "66"
}